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Release date:April 24, 2025 1:52 PM

Faculty of ScienceCreating species diversity: Analyzing the genes of organisms present in the harsh environments of alpine zones

Academic Assembly School of Science and Technology Institute of Science, ProfessorTOJO Koji

Alpine zones, such as the Chubu Mountains, are home to several organisms that have through evolution, adapted to harsh environments such as low temperatures and freezing. Professor Tojo and his team studied the evolutionary process of an aquatic insect known as Asynarchus sachalinensis at the genetic level and revealed that A. sachalinensis split into two groups that could now be considered “separate species,” as the glacial and interglacial periods repeated. The findings of this study indicate that climate changes in the past triggered the birth of a new species and that the environment of the alpine zones has promoted the evolution and diversification of life.

Analyzing the genes of the Asynarchus sachalinensis to explore its evolution and the effects of climate change

Preservation of ecosystems has emerged as one of the most important issues as global warming progresses. Glaciers and snowfields are shrinking rapidly, particularly in mountainous regions. Consequently, endemic organisms that have adapted to harsh environmental conditions such as low temperature, drought, and strong ultraviolet rays are at risk of extinction. Professor Tojo and his team analyzed the past evolutionary process of A. sachalinensis at a genetic level, an aquatic insect present in cool ponds and springs that can survive in cold environments. Furthermore, they also investigated the effects of past climate changes on the range shift of A. sachalinensis, the connectivity and fragmentation of local populations, and the resulting genetic structure.

Asynarchus sachalinensis split into two groups approximately 2 million years ago

A. sachalinensis is a species of caddisflies that is 1–1.5 cm long. A total of 144 specimens of A. sachalinensis were collected from 74 locations from the Chubu-Sangaku Mountain Range to Sakhalin. The base sequence (*2) of their entire genome DNA (*1) were obtained from parts of their walking legs. Analysis of multiple regions of mitochondrial DNA (*3) revealed that A. sachalinensis split into two groups (groups I and II) approximately 2 million years ago. Group I was widely distributed in the relatively lowlands of Hokkaido and Tohoku. Group II was scattered mainly in the alpine zone of central and western Honshu.

A. sachalinensis habitat, adults and larvae. (a) A typical sub-alpine habitat of Group I (Mt. Norikura, altitude approx. 1,200 m), (b) a typical alpine habitat of Group II(Mt. Norikura, altitude approx. 2,700 m), (c) male, (d) female, (e) larva and its case.

Climate change will give rise to new species! High altitudes support species diversification!

Genetic analysis revealed that “during the repeated glacial and interglacial periods, Group I existed at relatively low altitudes, allowing it to move easily and distribute widely, whereas Group II existed at high altitudes, resulting in a scattered distribution in isolated environments.” Furthermore, high-altitude organisms were more likely to acquire unique lifestyles (subject to natural selection) in the harsh and isolated environments unique to high altitudes. Subsequent research has also shown that although populations of both groups are present on Mt. Norikura, differences have been noted between alpine and sub-alpine zones of Mt. Norikura in terms of reproductive timing. An accumulation of genetic mutations has resulted in them coming to form “separate species.” Thus, it has demonstrated how climate change can lead to the birth of new species and that high altitudes, in particular, support species diversification.

Points

  • Professor Tojo and his team analyzed the evolutionary process of A. sachalinensis at a genetic level, an aquatic insect present in the harsh environments of alpine zones, to determine the influence of climate change on its evolution.

  • Analysis of mitochondrial DNA revealed that A. sachalinensis split into two groups approximately two million years ago, with Group I being distributed widely in lowlands and Group II being scattered in highlands.

  • Climate change can give rise to new species; organisms in harsh and isolated alpine zones are particularly susceptible to developing lifestyles that differ from those of the organisms in lowlands .

Keywords

*1 Whole genomic DNA

Deoxyribonucleic acid (DNA) contains all the genetic information of an organism, including nuclear DNA and mitochondrial DNA. It is the blueprint for living things.

*2 Nucleotide sequence

DNA comprises four bases: adenine, thymine, guanine, and cytosine. The base sequence refers to the order in which these four bases are arranged, which in turn determines the specific proteins that are synthesized.

*3 Mitochondrial DNA

Circular DNA is present in the mitochondria within cells. Sequence information of specific regions (e.g., COI and 16S rRNA) is often used for evolutionary phylogenetic analysis of organisms.

Paper

Journal:Ecology and Evolution. 2024; 14:e11428.
Title:Variations in the phenological patterns of a caddisfly inhabiting the same mountain massifs: Life‐history differences in different altitudinal zones.
Author:Hirohisa Suzuki, Masaki Takenaka, Koji Tojo

https://onlinelibrary.wiley.com/doi/10.1002/ece3.11428

Researcher

TOJO Koji

Affiliation

Academic Assembly School of Science and Technology Institute of Science, Professor

Link
https://soar-rd.shinshu-u.ac.jp/search/detail.html?systemId=OCcebhLh&lang=en
What led you to choose this research theme?

This research was made possible by Dr. Hirohisa Suzuki, the lead author of this paper, who developed the analytical methods used in our laboratory. He was a graduate student serving as a high school science teacher. Gaining new knowledge, despite the challenges of conducting fieldwork in high mountainous areas, with a tight and demanding schedule and conducting new genetic analysis, was a major achievement.

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