食品微生物工学研究室

信州大学農学部農学生命科学科
生命機能科学コース
食品微生物工学研究室
令和6年7月30日　メンバー

令和6年3月2日　池田正人先生最終講義

令和6年3月2日　OB・OG会（第6回）

最終更新日　令和6年9月30日

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研究分野


研究室メンバー　　　准教授　　　　竹野誠記　　　特任教授　　　池田正人	大学院生　修士2年　岡部　茉伊子　修士1年　青嶋　里央夢　高田　健吾　宮川　侑己	学部学生　学部4年　田所　一慶　森　彩華　横山　美久　学部3年　阿久津　皓平　藤田　貫伍　向本　千穂

研究テーマ

研究テーマの説明

発酵は生命の活動であり、そのしくみは未だ多くがベールに包まれています。これが「発酵は神秘」と言われる所以です。私たちは、その発酵の世界に「ゲノム科学」という最新のメスを入れ、発酵の全貌を科学的に解き明かしたいと思っています。そうすれば、新たな発見が生まれ、理論的な方法論が育ち、そして従来の発酵を大きく変えられる革新的な技術へとつながっていくと考えています。研究のアプローチとしては、第一に、発酵微生物の生理やゲノムを解析して、「発酵を成立させているしくみ」や「生理的特性を決定づけている遺伝子」を明らかにしていきます。第二に、それらの知見を応用した「代謝工学」と「生理工学」の方法論を開発していきます。また、細菌による脂質発酵の検討も始めます。非水溶性物質の発酵生産技術はカビや藻類が主体で、アミノ酸や核酸等、水溶性物質生産の主役である細菌はなぜかマイナーです。細菌による脂質発酵の課題を明らかにして、基盤となる技術を構築していきます。

最近の著書・発表論文

【1】	Takeno, S., Hirata, Y., Kitamura, K., Ohtake, T., Aoki, K., Murata, N., Hayashi, M., & Ikeda, M.：Metabolic engineering to produce palmitic acid or palmitoleic acid in an oleic acid-producing Corynebacterium glutamicum strain. Metab. Eng., 78: 148-158 (2023).
【2】	Ikeda, M., Takahashi, K., Ohtake, T., Imoto, R., Kawakami, H., Hayashi, M., & Takeno, S.：A Futile Metabolic Cycle of Fatty Acyl Coenzyme A (Acyl-CoA) Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production. Appl. Environ. Microbiol., 87(4): e02469-20 (2021).
【3】	Ikeda, M. & Takeno, S.：Recent Advances in Amino Acid Production, In: Inui, M. & Toyoda, K. (eds), Corynebacterium glutamicum -Biology and Biotechnology-, 2nd edition. Springer Nature Switzerland AG, Cham, Switzerland, pp.175-226 (2020).
【4】	Takeno, S., Murata, N., Kura, M., Takasaki, M., Hayashi, M., & Ikeda, M.：The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains. Appl. Microbiol. Biotechnol., 102(24): 10603-10612 (2018).
【5】	Ikeda, M., Nagashima, T., Nakamura, E., Kato, R., Ohshita, M., Hayashi, M., & Takeno, S.：In vivo roles of fatty acid biosynthesis enzymes in biosynthesis of biotin and α-lipoic acid in Corynebacterium glutamicum. Appl. Environ. Microbiol., 83(19): e01322-17 (2017).
【6】	Yokota, A. & Ikeda, M. (Editors)：Amino Acid Fermentation. Adv. Biochem. Eng. Biotechnol., 159, Springer (2017).
【7】	Takeno, S., Hori, K., Ohtani, S., Mimura, A., Mitsuhashi, S., & Ikeda, M.：L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Metab. Eng., 37:1-10 (2016).
【8】	Ikeda, M., Noguchi, N., Ohshita, M., Senoo, A., Mitsuhashi, S., & Takeno, S.：A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833. Appl. Microbiol. Biotechnol., 99:2741-2750 (2015).
【9】	Takeno, S., Takasaki, M., Urabayashi, A., Mimura, A., Muramatsu, T., Mitsuhashi, S., & Ikeda, M.：Development of fatty acid-producing Corynebacterium glutamicum strains. Appl. Environ. Microbiol., 79:6776-6783 (2013).
【10】	Ikeda, M., Miyamoto, A., Mutoh, S., Kitano, Y., Tajima, M., Shirakura, D., Takasaki, M., Mitsuhashi, S., & Takeno, S.：Development of biotin-prototrophic and -hyperauxotrophic Corynebacterium glutamicum strains. Appl. Environ. Microbiol., 79:4586-4594 (2013).
【11】	Takeno, S., Shirakura, D., Tsukamoto, N., Mitsuhashi, S., & Ikeda, M.：Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 97:1259-1267 (2013).
【12】	Ikeda, M. & Takeno, S.：Amino Acid Production by Corynebacterium glutamicum, In: Yukawa, H. & Inui, M. (eds), Corynebacterium glutamicum -Biology and Biotechnology-. Springer-Verlag, Berlin, Heidelberg, pp.107-147 (2013).
【13】	Ikeda, M.：Sugar transport systems in Corynebacterium glutamicum: features and applications to strain development. Appl. Microbiol. Biotechnol., 96:1191-1200 (2012).
【14】	Ikeda, M., Mizuno, Y., Awane, S., Hayashi, M., Mitsuhashi, S., & Takeno, S.：Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 90:1443-1451 (2011).
【15】	Takeno, S., Murata, R., Kobayashi, R., Mitsuhashi, S., & Ikeda, M.：Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production. Appl. Environ. Microbiol., 76:7154-7160 (2010).
【16】	Ikeda, M. & Takeno, S. ：Genetic Engineering of Corynebacteria, In: Baltz, R. H., Davies, J. E. & Demain, A. L. (eds), Manual of Industrial Microbiology and Biotechnology, 3rd Edition. ASM Press, Washington DC, pp.225-237 (2010).
【17】	Ikeda, M., Baba, M., Tsukamoto, N., Komatsu, T., Mitsuhashi, S., & Takeno, S.：Elucidation of genes relevant to the microaerobic growth of Corynebacterium glutamicum. Biosci. Biotechnol. Biochem., 73:2806-2808 (2009).
【18】	Ikeda, M., Mitsuhashi, S., Tanaka, K., & Hayashi, M.：Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl. Environ. Microbiol., 75:1635-1641 (2009).
【19】	Takeno, S., Nakamura, M., Fukai R., Ohnishi, J., & Ikeda, M.：The Cgl1281-encoding putative transporter of the cation diffussion facilitator family is responsible for alkali-tolerance in Corynebacterium glutamicum. Arch. Microbiol., 190:531-538 (2008).
【20】	Ohnishi, J., Mizoguchi, H., Takeno, S., & Ikeda, M.：Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain. Mutat. Res., 649:239-244 (2008).
【21】	Takeno, S., Ohnishi, J., Komatsu, T., Masaki, T., Sen, K., & Ikeda, M.：Anaerobic Growth and Potential for Amino Acid Production by Nitrate Respiration in Corynebacterium glutamicum, Appl. Microbiol. Biotechnol., 75:1173-1182 (2007).
【22】	Mitsuhashi, S., Hayashi, M., Ohnishi, J., & Ikeda, M.：Disruption of Malate:Quinone Oxidoreductase Increases L-Lysine Production by Corynebacterium glutamicum, Biosci. Biotechnol. Biochem., 70:2803-2806 (2006).
【23】	Ikeda, M., Ohnishi, J., Hayashi, M., & Mitsuhashi, S.：A genome-Based Approach to Create a Minimally Mutated Corynebacterium glutamicum Strain for Efficient L-Lysine Production, J. Ind. Microbiol. Biotechnol., 33:610-615 (2006).
【24】	Ikeda, M.：Towards Bacterial Strains Overproducing L-Tryptophan and Other Aromatics by Metabolic Engineering, Appl. Microbiol. Biotechnol., 69:615-626 (2006).
【25】	Sakuradani, E., Takeno, S., Abe, T., Ogawa, J. & Shimizu, S.：Arachidonic Acid-Producing Mortierella alpina: Molecular Breeding of Mutants and Creation and Application of a Host-Vector System, In: Hou, C. T. & Shaw, J. (eds), Biocatalysis and Biotechnology for Functional Foods and Industrial Products. CRC Press, Boca Raton, Florida, pp.267-282 (2006).
【26】	Hayashi, M., Mizoguchi, H., Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., & Ikeda, M.：A leuC Mutation Leading to Increased L-Lysine Production and rel-Independent Global Expression Changes in Corynebacterium glutamicum”, Appl. Microbiol. Biotechnol., 72:783-789 (2006).
【27】	Hayashi, M, Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., & Ikeda, M.： Transcriptome Analysis Reveals Global Expression Changes in an Industrial L-Lysine producer of Corynebacterium glutamicum, Biosci. Biotech. Biochem., 70:546-550 (2006).
【28】	Ohnishi, J. & Ikeda, M.：Comparisons of Potentials for L-Lysine Production among Different Corynebacterium glutamicum Strains, Biosci. Biotech. Biochem., 70:1017-1020 (2006).
【29】	Takeno, S., Sakuradani, E., Tomi, A., Inohara-Ochiai, M., Kawashima, H., & Shimizu, S.：Transformation of Oil-Producing Fungus, Mortierella alpina 1S-4, Using Zeocin, and Application to Arachidonic Acid Production, J. Biosci. Bioeng., 100:617-622 (2005).
【30】	Ikeda, M, Ohnishi, J., & Mitsuhashi, S.：Genome Breeding of an Amino Acid-producing Corynebacterium glutamicum Mutant, In: Barredo, J. L. S. (ed) Microbial Processes and Products. Humana Press, Totowa, pp 179-189 (2005).
【31】	Ikeda, M.：Tryptophan Production. In: Eggeling, L. & Bott, M. (eds) Handbook of Corynebacterium glutamicum. CRC Press LLC, Boca Raton, pp 489-509 (2005).
【32】	Ohnishi, J., Katahira, R,Mitsuhashi, S., Kakita, S., & Ikeda, M.：A Novel gnd Mutation Leading to Increased L-Lysine Production in Corynebacterium glutamicum, FEMS Microbiol. Lett. 242: 265-274 (2005).
【33】	Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Molecular Evidence That the Rate-Limiting Step for the Biosynthesis of Arachidonic Acid in Mortierella alpina Is at the Level of an Elongase, Lipids, 40: 25-30 (2005).
【34】	Sakuradani, E., Takeno, S., Abe, T., & Shimizu, S.：Arachidonic Acid-Producing Mortierella alpina: Creation of Mutants and Molecular Breeding. In: Cohen, Z. & Ratledge, C. (eds), Single Cell Oils. AOCS Press, Champaign, Illinois, pp.21-35 (2005).
【35】	Takeno, S., Sakuradani, E., Tomi, A., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Improvement of the Fatty Acid Composition of an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, through RNA Interference with D12-Desaturase Gene Expression, Appl. Environ. Microbiol., 71: 5124-5128 (2005).
【36】	Mitsuhashi, S., Ohnishi, J., Hayashi, M., & Ikeda, M.：A Gene Homologous to beta-type Carbonic Anhydrase is Essential for Growth of Corynebacterium glutamicum under Atmospheric Conditions, Appl. Microbiol. Biotechnol., 63: 592-601 (2004).
【37】	Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4, Biosci. Biotechnol. Biochem., 68: 277-285 (2004).
【38】	Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Establishment of an Overall Transformation System for an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, Appl. Microbiol. Biotechnol., 65: 419-425 (2004).
【39】	Ikeda, M. & Nakagawa, S.：The Corynebacterium glutamicum Genome: Features and Impacts on Biotechnological Processes, Appl. Microbiol. Biotechnol., 62: 99-109 (2003).
【40】	Ikeda, M.：Amino Acid Production Processes. In: Faurie R, Thommel J (eds), Adv. Biochem. Eng. Biotechnol. vol. 79. Microbial Production of L-Amino Acids. Springer-Verlag, Berlin, Heidelberg, pp.1-35 (2003).
【41】	Ohnishi, J., Hayashi, M., Mitsuhashi, S., & Ikeda, M.：Efficient 40 degrees C Fermentation of L-Lysine by a New Corynebacterium glutamicum Mutant Developed by Genome Breeding, Appl. Microbiol. Biotechnol., 62: 69-75 (2003).
【42】	Kamada, N., Yasuhara, A., & Ikeda, M.：Significance of the Non-oxidative Route of the Pentose Phosphate Pathway for Supplying Carbon to the Purine-nucleotide Pathway in Corynebacterium ammoniagenes, J. Ind. Microbiol. Biotechnol., 30: 129-132 (2003).
【43】	Hayashi, M., Mizoguchi, H., Shiraishi, N., Obayashi, M., Nakagawa, S., Imai, J., Watanabe, S., Ota, T., & Ikeda, M.：Transcriptome Analysis of Acetate Metabolism in Corynebacterium glutamicum Using a Newly Developed Metabolic Array, Biosci. Biotech. Biochem., 66: 1337-1344 (2002).
【44】	Ohnishi, J., Mitsuhashi, S., Hayashi, M., Ando, S., Yokoi, H., Ochiai, K., & Ikeda, M.：A Novel Methodology of Employing Corynebacterium glutamicum Genome Information to Generate a New L-Lysine-producing Mutant, Appl. Microbiol. Biotechnol., 58: 217-223 (2002).
【45】	Kamada, N., Yasuhara, A., Takano, Y., Nakano, T., & Ikeda, M.：Effect of Transketolase Modifications on Carbon Flow to the Purine-nucleotide Pathway in Corynebacterium ammoniagenes, Appl. Microbiol. Biotechnol., 56: 710-717 (2001).

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