Laboratory of Food Microbial Engineering

Laboratory of Food Microbial Engineering
Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences,
Faculty of Agriculture, Shinshu University

Departure (Mar. 23, 2024)

The last lecture by Prof. Masato Ikeda (Mar. 2, 2024)

Reunion (Mar. 2, 2024)

Last update: Apr. 1, 2024

Japanese

Field of Research
Group Members
Research Theme
Further Information
Publications
Link

Field of Research

Group Members

Associate Professor
Seiki TAKENO, Ph.D.

Specially Appointed Professor
Masato IKEDA, Ph.D.

Graduate Students

　M2

　Maiko OKABE

　M1

　Rion AOSHIMA
　Yuki MIYAKAWA
　Kengo TAKADA

Undergraduate Students

　B4

　Ayaka MORI
　Ikkei TADOKORO
　Miku YOKOYAMA

Research Theme

Further Information

Fermentation is ‘activity of life’ itself, and thus, is veiled in mystery. Much remains to be elucidated. Our aim is to clarify the mystery of fermentation with the help of genomics. Then, hitherto unknown mechanisms for fermentation may emerge, which will provide new and valuable insights into strategies of strain development and will lead to the exploitation of innovative fermentation processes. Toward this goal, we will firstly investigate the physiology and genome of producer organisms to address the molecular mechanism of high-level production and also the genetic basis restricting the performance of the organisms. Secondly, we will apply the information to extend the performance of producer strains as well as to develop new methodology not only for ‘metabolic engineering’ but also for ‘physiologic engineering’. We will also direct our eyes not only to water-soluble metabolites but also to water-non-soluble lipid for the development of bacterial strains excreting lipid.

Publications

【1】
　　Takeno, S., Hirata, Y., Kitamura, K., Ohtake, T., Aoki, K., Murata, N., Hayashi, M., & Ikeda, M.：Metabolic engineering to produce palmitic acid or palmitoleic acid in an oleic acid-producing Corynebacterium glutamicum strain. Metab. Eng., 78: 148-158 (2023).

【2】
　　Ikeda, M., Takahashi, K., Ohtake, T., Imoto, R., Kawakami, H., Hayashi, M., & Takeno, S.：A Futile Metabolic Cycle of Fatty Acyl Coenzyme A (Acyl-CoA) Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production. Appl. Environ. Microbiol., 87(4): e02469-20 (2021).

【3】
　　Ikeda, M. & Takeno, S.：Recent Advances in Amino Acid Production, In: Inui, M. & Toyoda, K. (eds), Corynebacterium glutamicum -Biology and Biotechnology-, 2nd edition. Springer Nature Switzerland AG, Cham, Switzerland, pp.175-226 (2020).

【4】
　　Takeno, S., Murata, N., Kura, M., Takasaki, M., Hayashi, M., & Ikeda, S.：The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains. Appl. Microbiol. Biotechnol., 102(24): 10603-10612 (2018).

【5】
　　Ikeda, M., Nagashima, T., Nakamura, E., Kato, R., Ohshita, M., Hayashi, M., & Takeno, S.：In vivo roles of fatty acid biosynthesis enzymes in biosynthesis of biotin and α-lipoic acid in Corynebacterium glutamicum. Appl. Environ. Microbiol., 83(19): e01322-17 (2017).

【6】
　　Yokota, A. & Ikeda, M. (Editors)：Amino Acid Fermentation. Adv. Biochem. Eng. Biotechnol., 159, Springer (2017).

【7】
　　Takeno, S., Hori, K., Ohtani, S., Mimura, A., Mitsuhashi, S., & Ikeda, M.：L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Metab. Eng., 37:1-10 (2016).

【8】
　　Ikeda, M., Noguchi, N., Ohshita, M., Senoo, A., Mitsuhashi, S., & Takeno, S.：A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833. Appl. Microbiol. Biotechnol., 99:2741-2750 (2015).

【9】
　　Takeno, S., Takasaki, M., Urabayashi, A., Mimura, A., Muramatsu, T., Mitsuhashi, S., & Ikeda, M.：Development of fatty acid-producing Corynebacterium glutamicum strains. Appl. Environ. Microbiol., 79:6776-6783 (2013).

【10】
　　Ikeda, M., Miyamoto, A., Mutoh, S., Kitano, Y., Tajima, M., Shirakura, D., Takasaki, M., Mitsuhashi, S., & Takeno, S.：Development of biotin-prototrophic and -hyperauxotrophic Corynebacterium glutamicum strains. Appl. Environ. Microbiol., 79:4586-4594 (2013).

【11】
　　Takeno, S., Shirakura, D., Tsukamoto, N., Mitsuhashi, S., & Ikeda, M.：Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 97:1259-1267 (2013).

【12】
　　Ikeda, M. & Takeno, S.：Amino Acid Production by Corynebacterium glutamicum, In: Yukawa, H. & Inui, M. (eds), Corynebacterium glutamicum -Biology and Biotechnology-. Springer-Verlag, Berlin, Heidelberg, pp.107-147 (2013).

【13】
　　Ikeda, M.：Sugar transport systems in Corynebacterium glutamicum: features and applications to strain development. Appl. Microbiol. Biotechnol., 96:1191-1200 (2012).

【14】
　　Ikeda, M., Mizuno, Y., Awane, S., Hayashi, M., Mitsuhashi, S., & Takeno, S.：Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 90:1443-1451 (2011).

【15】
　　Takeno, S., Murata, R., Kobayashi, R., Mitsuhashi, S., & Ikeda, M.：Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production. Appl. Environ. Microbiol., 76:7154-7160 (2010).

【16】
　　Ikeda, M. & Takeno, S. ：Genetic Engineering of Corynebacteria, In: Baltz, R. H., Davies, J. E. & Demain, A. L. (eds), Manual of Industrial Microbiology and Biotechnology, 3rd Edition. ASM Press, Washington DC, pp.225-237 (2010).

【17】
　　Ikeda, M., Baba, M., Tsukamoto, N., Komatsu, T., Mitsuhashi, S., & Takeno, S.：Elucidation of genes relevant to the microaerobic growth of Corynebacterium glutamicum. Biosci. Biotechnol. Biochem., 73:2806-2808 (2009).

【18】
　　Ikeda, M., Mitsuhashi, S., Tanaka, K., & Hayashi, M.：Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl. Environ. Microbiol., 75:1635-1641 (2009).

【19】
　　Takeno, S., Nakamura, M., Fukai R., Ohnishi, J., & Ikeda, M.：The Cgl1281-encoding putative transporter of the cation diffussion facilitator family is responsible for alkali-tolerance in Corynebacterium glutamicum. Arch. Microbiol., 190:531-538 (2008).

【20】
　　Ohnishi, J., Mizoguchi, H., Takeno, S., & Ikeda, M.：Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain. Mutat. Res., 649:239-244 (2008).

【21】　　Takeno, S., Ohnishi, J., Komatsu, T., Masaki, T., Sen, K., & Ikeda, M.：Anaerobic Growth and Potential for Amino Acid Production by Nitrate Respiration in Corynebacterium glutamicum, Appl. Microbiol. Biotechnol., 75:1173-1182 (2007).

【22】
　Mitsuhashi, S., Hayashi, M., Ohnishi, J., & Ikeda, M.：Disruption of Malate:Quinone Oxidoreductase Increases L-Lysine Production by Corynebacterium glutamicum, Biosci. Biotechnol. Biochem., 70:2803-2806 (2006).

【23】　　Ikeda, M., Ohnishi, J., Hayashi, M., & Mitsuhashi, S.：A genome-Based Approach to Create a Minimally Mutated Corynebacterium glutamicum Strain for Efficient L-Lysine Production, J. Ind. Microbiol. Biotechnol., 33:610-615 (2006).

【24】
　　Ikeda, M.：Towards Bacterial Strains Overproducing L-Tryptophan and Other Aromatics by Metabolic Engineering, Appl. Microbiol. Biotechnol., 69:615-626 (2006).

【25】
　　Sakuradani, E., Takeno, S., Abe, T., Ogawa, J. & Shimizu, S.：Arachidonic Acid-Producing Mortierella alpina: Molecular Breeding of Mutants and Creation and Application of a Host-Vector System, In: Hou, C. T. & Shaw, J. (eds), Biocatalysis and Biotechnology for Functional Foods and Industrial Products. CRC Press, Boca Raton, Florida, pp.267-282 (2006).

【26】　　Hayashi, M., Mizoguchi, H., Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., & Ikeda, M.：A leuC Mutation Leading to Increased L-Lysine Production and rel-Independent Global Expression Changes in Corynebacterium glutamicum”, Appl. Microbiol. Biotechnol., 72:783-789 (2006).

【27】　　Hayashi, M, Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., & Ikeda, M.： Transcriptome Analysis Reveals Global Expression Changes in an Industrial L-Lysine producer of Corynebacterium glutamicum, Biosci. Biotech. Biochem., 70:546-550 (2006).

【28】　　Ohnishi, J. & Ikeda, M.：Comparisons of Potentials for L-Lysine Production among Different Corynebacterium glutamicum Strains, Biosci. Biotech. Biochem., 70:1017-1020 (2006).

【29】　　Takeno, S., Sakuradani, E., Tomi, A., Inohara-Ochiai, M., Kawashima, H., & Shimizu, S.：Transformation of Oil-Producing Fungus, Mortierella alpina 1S-4, Using Zeocin, and Application to Arachidonic Acid Production, J. Biosci. Bioeng., 100:617-622 (2005).

【30】　　Ikeda, M, Ohnishi, J., & Mitsuhashi, S.：Genome Breeding of an Amino Acid-producing Corynebacterium glutamicum Mutant, In: Barredo, J. L. S. (ed) Microbial Processes and Products. Humana Press, Totowa, pp 179-189 (2005).

【31】　　Ikeda, M.：Tryptophan Production. In: Eggeling, L. & Bott, M. (eds) Handbook of Corynebacterium glutamicum. CRC Press LLC, Boca Raton, pp 489-509 (2005).

【32】　　Ohnishi, J., Katahira, R,Mitsuhashi, S., Kakita, S., & Ikeda, M.：A Novel gnd Mutation Leading to Increased L-Lysine Production in Corynebacterium glutamicum, FEMS Microbiol. Lett. 242: 265-274 (2005).

【33】　　Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Molecular Evidence That the Rate-Limiting Step for the Biosynthesis of Arachidonic Acid in Mortierella alpina Is at the Level of an Elongase, Lipids, 40: 25-30 (2005).

【34】　　Sakuradani, E., Takeno, S., Abe, T., & Shimizu, S.：Arachidonic Acid-Producing Mortierella alpina: Creation of Mutants and Molecular Breeding. In: Cohen, Z. & Ratledge, C. (eds), Single Cell Oils. AOCS Press, Champaign, Illinois, pp.21-35 (2005).

【35】　　Takeno, S., Sakuradani, E., Tomi, A., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Improvement of the Fatty Acid Composition of an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, through RNA Interference with D12-Desaturase Gene Expression, Appl. Environ. Microbiol., 71: 5124-5128 (2005).

【36】　　Mitsuhashi, S., Ohnishi, J., Hayashi, M., & Ikeda, M.：A Gene Homologous to beta-type Carbonic Anhydrase is Essential for Growth of Corynebacterium glutamicum under Atmospheric Conditions, Appl. Microbiol. Biotechnol., 63: 592-601 (2004).

【37】　　Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4, Biosci. Biotechnol. Biochem., 68: 277-285 (2004).

【38】　　Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Establishment of an Overall Transformation System for an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, Appl. Microbiol. Biotechnol., 65: 419-425 (2004).

【39】　　Ikeda, M. & Nakagawa, S.：The Corynebacterium glutamicum Genome: Features and Impacts on Biotechnological Processes, Appl. Microbiol. Biotechnol., 62: 99-109 (2003).

【40】　　Ikeda, M.：Amino Acid Production Processes. In: Faurie R, Thommel J (eds), Adv. Biochem. Eng. Biotechnol. vol. 79. Microbial Production of L-Amino Acids. Springer-Verlag, Berlin, Heidelberg, pp.1-35 (2003).

【41】　　Ohnishi, J., Hayashi, M., Mitsuhashi, S., & Ikeda, M.：Efficient 40 degrees C Fermentation of L-Lysine by a New Corynebacterium glutamicum Mutant Developed by Genome Breeding, Appl. Microbiol. Biotechnol., 62: 69-75 (2003).

【42】　　Kamada, N., Yasuhara, A., & Ikeda, M.：Significance of the Non-oxidative Route of the Pentose Phosphate Pathway for Supplying Carbon to the Purine-nucleotide Pathway in Corynebacterium ammoniagenes, J. Ind. Microbiol. Biotechnol., 30: 129-132 (2003).

【43】　　Hayashi, M., Mizoguchi, H., Shiraishi, N., Obayashi, M., Nakagawa, S., Imai, J., Watanabe, S., Ota, T., & Ikeda, M.：Transcriptome Analysis of Acetate Metabolism in Corynebacterium glutamicum Using a Newly Developed Metabolic Array, Biosci. Biotech. Biochem., 66: 1337-1344 (2002).

【44】　　Ohnishi, J., Mitsuhashi, S., Hayashi, M., Ando, S., Yokoi, H., Ochiai, K., & Ikeda, M.：A Novel Methodology of Employing Corynebacterium glutamicum Genome Information to Generate a New L-Lysine-producing Mutant, Appl. Microbiol. Biotechnol., 58: 217-223 (2002).

【45】　　Kamada, N., Yasuhara, A., Takano, Y., Nakano, T., & Ikeda, M.：Effect of Transketolase Modifications on Carbon Flow to the Purine-nucleotide Pathway in Corynebacterium ammoniagenes, Appl. Microbiol. Biotechnol., 56: 710-717 (2001).

Link

Department of Agricultural and Life Sciences, Faculty of Agriculture, Shinshu University
Faculty of Agriculture, Shinshu University

Laboratory of Food Microbial Engineering,
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University
8304 Minami-minowa, Nagano 399-4598, JAPAN

Copyright (c) Laboratory of Food Microbial Engineering All Rights Reserved.

【1】	Takeno, S., Hirata, Y., Kitamura, K., Ohtake, T., Aoki, K., Murata, N., Hayashi, M., & Ikeda, M.：Metabolic engineering to produce palmitic acid or palmitoleic acid in an oleic acid-producing Corynebacterium glutamicum strain. Metab. Eng., 78: 148-158 (2023).
【2】	Ikeda, M., Takahashi, K., Ohtake, T., Imoto, R., Kawakami, H., Hayashi, M., & Takeno, S.：A Futile Metabolic Cycle of Fatty Acyl Coenzyme A (Acyl-CoA) Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production. Appl. Environ. Microbiol., 87(4): e02469-20 (2021).
【3】	Ikeda, M. & Takeno, S.：Recent Advances in Amino Acid Production, In: Inui, M. & Toyoda, K. (eds), Corynebacterium glutamicum -Biology and Biotechnology-, 2nd edition. Springer Nature Switzerland AG, Cham, Switzerland, pp.175-226 (2020).
【4】	Takeno, S., Murata, N., Kura, M., Takasaki, M., Hayashi, M., & Ikeda, S.：The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains. Appl. Microbiol. Biotechnol., 102(24): 10603-10612 (2018).
【5】	Ikeda, M., Nagashima, T., Nakamura, E., Kato, R., Ohshita, M., Hayashi, M., & Takeno, S.：In vivo roles of fatty acid biosynthesis enzymes in biosynthesis of biotin and α-lipoic acid in Corynebacterium glutamicum. Appl. Environ. Microbiol., 83(19): e01322-17 (2017).
【6】	Yokota, A. & Ikeda, M. (Editors)：Amino Acid Fermentation. Adv. Biochem. Eng. Biotechnol., 159, Springer (2017).
【7】	Takeno, S., Hori, K., Ohtani, S., Mimura, A., Mitsuhashi, S., & Ikeda, M.：L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Metab. Eng., 37:1-10 (2016).
【8】	Ikeda, M., Noguchi, N., Ohshita, M., Senoo, A., Mitsuhashi, S., & Takeno, S.：A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833. Appl. Microbiol. Biotechnol., 99:2741-2750 (2015).
【9】	Takeno, S., Takasaki, M., Urabayashi, A., Mimura, A., Muramatsu, T., Mitsuhashi, S., & Ikeda, M.：Development of fatty acid-producing Corynebacterium glutamicum strains. Appl. Environ. Microbiol., 79:6776-6783 (2013).
【10】	Ikeda, M., Miyamoto, A., Mutoh, S., Kitano, Y., Tajima, M., Shirakura, D., Takasaki, M., Mitsuhashi, S., & Takeno, S.：Development of biotin-prototrophic and -hyperauxotrophic Corynebacterium glutamicum strains. Appl. Environ. Microbiol., 79:4586-4594 (2013).
【11】	Takeno, S., Shirakura, D., Tsukamoto, N., Mitsuhashi, S., & Ikeda, M.：Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 97:1259-1267 (2013).
【12】	Ikeda, M. & Takeno, S.：Amino Acid Production by Corynebacterium glutamicum, In: Yukawa, H. & Inui, M. (eds), Corynebacterium glutamicum -Biology and Biotechnology-. Springer-Verlag, Berlin, Heidelberg, pp.107-147 (2013).
【13】	Ikeda, M.：Sugar transport systems in Corynebacterium glutamicum: features and applications to strain development. Appl. Microbiol. Biotechnol., 96:1191-1200 (2012).
【14】	Ikeda, M., Mizuno, Y., Awane, S., Hayashi, M., Mitsuhashi, S., & Takeno, S.：Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 90:1443-1451 (2011).
【15】	Takeno, S., Murata, R., Kobayashi, R., Mitsuhashi, S., & Ikeda, M.：Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production. Appl. Environ. Microbiol., 76:7154-7160 (2010).
【16】	Ikeda, M. & Takeno, S. ：Genetic Engineering of Corynebacteria, In: Baltz, R. H., Davies, J. E. & Demain, A. L. (eds), Manual of Industrial Microbiology and Biotechnology, 3rd Edition. ASM Press, Washington DC, pp.225-237 (2010).
【17】	Ikeda, M., Baba, M., Tsukamoto, N., Komatsu, T., Mitsuhashi, S., & Takeno, S.：Elucidation of genes relevant to the microaerobic growth of Corynebacterium glutamicum. Biosci. Biotechnol. Biochem., 73:2806-2808 (2009).
【18】	Ikeda, M., Mitsuhashi, S., Tanaka, K., & Hayashi, M.：Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl. Environ. Microbiol., 75:1635-1641 (2009).
【19】	Takeno, S., Nakamura, M., Fukai R., Ohnishi, J., & Ikeda, M.：The Cgl1281-encoding putative transporter of the cation diffussion facilitator family is responsible for alkali-tolerance in Corynebacterium glutamicum. Arch. Microbiol., 190:531-538 (2008).
【20】	Ohnishi, J., Mizoguchi, H., Takeno, S., & Ikeda, M.：Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain. Mutat. Res., 649:239-244 (2008).
【21】	Takeno, S., Ohnishi, J., Komatsu, T., Masaki, T., Sen, K., & Ikeda, M.：Anaerobic Growth and Potential for Amino Acid Production by Nitrate Respiration in Corynebacterium glutamicum, Appl. Microbiol. Biotechnol., 75:1173-1182 (2007).
【22】	Mitsuhashi, S., Hayashi, M., Ohnishi, J., & Ikeda, M.：Disruption of Malate:Quinone Oxidoreductase Increases L-Lysine Production by Corynebacterium glutamicum, Biosci. Biotechnol. Biochem., 70:2803-2806 (2006).
【23】	Ikeda, M., Ohnishi, J., Hayashi, M., & Mitsuhashi, S.：A genome-Based Approach to Create a Minimally Mutated Corynebacterium glutamicum Strain for Efficient L-Lysine Production, J. Ind. Microbiol. Biotechnol., 33:610-615 (2006).
【24】	Ikeda, M.：Towards Bacterial Strains Overproducing L-Tryptophan and Other Aromatics by Metabolic Engineering, Appl. Microbiol. Biotechnol., 69:615-626 (2006).
【25】	Sakuradani, E., Takeno, S., Abe, T., Ogawa, J. & Shimizu, S.：Arachidonic Acid-Producing Mortierella alpina: Molecular Breeding of Mutants and Creation and Application of a Host-Vector System, In: Hou, C. T. & Shaw, J. (eds), Biocatalysis and Biotechnology for Functional Foods and Industrial Products. CRC Press, Boca Raton, Florida, pp.267-282 (2006).
【26】	Hayashi, M., Mizoguchi, H., Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., & Ikeda, M.：A leuC Mutation Leading to Increased L-Lysine Production and rel-Independent Global Expression Changes in Corynebacterium glutamicum”, Appl. Microbiol. Biotechnol., 72:783-789 (2006).
【27】	Hayashi, M, Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., & Ikeda, M.： Transcriptome Analysis Reveals Global Expression Changes in an Industrial L-Lysine producer of Corynebacterium glutamicum, Biosci. Biotech. Biochem., 70:546-550 (2006).
【28】	Ohnishi, J. & Ikeda, M.：Comparisons of Potentials for L-Lysine Production among Different Corynebacterium glutamicum Strains, Biosci. Biotech. Biochem., 70:1017-1020 (2006).
【29】	Takeno, S., Sakuradani, E., Tomi, A., Inohara-Ochiai, M., Kawashima, H., & Shimizu, S.：Transformation of Oil-Producing Fungus, Mortierella alpina 1S-4, Using Zeocin, and Application to Arachidonic Acid Production, J. Biosci. Bioeng., 100:617-622 (2005).
【30】	Ikeda, M, Ohnishi, J., & Mitsuhashi, S.：Genome Breeding of an Amino Acid-producing Corynebacterium glutamicum Mutant, In: Barredo, J. L. S. (ed) Microbial Processes and Products. Humana Press, Totowa, pp 179-189 (2005).
【31】	Ikeda, M.：Tryptophan Production. In: Eggeling, L. & Bott, M. (eds) Handbook of Corynebacterium glutamicum. CRC Press LLC, Boca Raton, pp 489-509 (2005).
【32】	Ohnishi, J., Katahira, R,Mitsuhashi, S., Kakita, S., & Ikeda, M.：A Novel gnd Mutation Leading to Increased L-Lysine Production in Corynebacterium glutamicum, FEMS Microbiol. Lett. 242: 265-274 (2005).
【33】	Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Molecular Evidence That the Rate-Limiting Step for the Biosynthesis of Arachidonic Acid in Mortierella alpina Is at the Level of an Elongase, Lipids, 40: 25-30 (2005).
【34】	Sakuradani, E., Takeno, S., Abe, T., & Shimizu, S.：Arachidonic Acid-Producing Mortierella alpina: Creation of Mutants and Molecular Breeding. In: Cohen, Z. & Ratledge, C. (eds), Single Cell Oils. AOCS Press, Champaign, Illinois, pp.21-35 (2005).
【35】	Takeno, S., Sakuradani, E., Tomi, A., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Improvement of the Fatty Acid Composition of an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, through RNA Interference with D12-Desaturase Gene Expression, Appl. Environ. Microbiol., 71: 5124-5128 (2005).
【36】	Mitsuhashi, S., Ohnishi, J., Hayashi, M., & Ikeda, M.：A Gene Homologous to beta-type Carbonic Anhydrase is Essential for Growth of Corynebacterium glutamicum under Atmospheric Conditions, Appl. Microbiol. Biotechnol., 63: 592-601 (2004).
【37】	Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4, Biosci. Biotechnol. Biochem., 68: 277-285 (2004).
【38】	Takeno, S., Sakuradani, E., Murata, S., Inohara-Ochiai, M., Kawashima, H., Ashikari, T., & Shimizu, S.：Establishment of an Overall Transformation System for an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, Appl. Microbiol. Biotechnol., 65: 419-425 (2004).
【39】	Ikeda, M. & Nakagawa, S.：The Corynebacterium glutamicum Genome: Features and Impacts on Biotechnological Processes, Appl. Microbiol. Biotechnol., 62: 99-109 (2003).
【40】	Ikeda, M.：Amino Acid Production Processes. In: Faurie R, Thommel J (eds), Adv. Biochem. Eng. Biotechnol. vol. 79. Microbial Production of L-Amino Acids. Springer-Verlag, Berlin, Heidelberg, pp.1-35 (2003).
【41】	Ohnishi, J., Hayashi, M., Mitsuhashi, S., & Ikeda, M.：Efficient 40 degrees C Fermentation of L-Lysine by a New Corynebacterium glutamicum Mutant Developed by Genome Breeding, Appl. Microbiol. Biotechnol., 62: 69-75 (2003).
【42】	Kamada, N., Yasuhara, A., & Ikeda, M.：Significance of the Non-oxidative Route of the Pentose Phosphate Pathway for Supplying Carbon to the Purine-nucleotide Pathway in Corynebacterium ammoniagenes, J. Ind. Microbiol. Biotechnol., 30: 129-132 (2003).
【43】	Hayashi, M., Mizoguchi, H., Shiraishi, N., Obayashi, M., Nakagawa, S., Imai, J., Watanabe, S., Ota, T., & Ikeda, M.：Transcriptome Analysis of Acetate Metabolism in Corynebacterium glutamicum Using a Newly Developed Metabolic Array, Biosci. Biotech. Biochem., 66: 1337-1344 (2002).
【44】	Ohnishi, J., Mitsuhashi, S., Hayashi, M., Ando, S., Yokoi, H., Ochiai, K., & Ikeda, M.：A Novel Methodology of Employing Corynebacterium glutamicum Genome Information to Generate a New L-Lysine-producing Mutant, Appl. Microbiol. Biotechnol., 58: 217-223 (2002).
【45】	Kamada, N., Yasuhara, A., Takano, Y., Nakano, T., & Ikeda, M.：Effect of Transketolase Modifications on Carbon Flow to the Purine-nucleotide Pathway in Corynebacterium ammoniagenes, Appl. Microbiol. Biotechnol., 56: 710-717 (2001).