44Wild type hkt1;1 mutant Outlook for researchreproductiveOutlook for students after graduationGraduates are employed by pharmaceutical andfood-related companies as well as national and localorganizations (as public officials).In what hasbecome a recent trend, some skilled graduates workatpotentialembryologists.infertilityhumanOutlook for researchI expect that my lab’s research could become animportant element in avoiding food and energyshortages that are expected in the near future ifwe can apply knowledge obtained from basicplant science to breed salt-tolerant plants.Outlook for students after graduationMany of the students in my lab choose topursue careers with companies whose workinvolves plants. Graduates also tend to beinterested in working forthatdevelop distinctive environmental technologiesor in becoming teachers.Department ofApplied BiologyDepartment ofApplied BiologyProfessorHOCHI ShinichiProfessor Hochi took his current position in2008 after working as a researcher (1986 to1992) at Snow Brand Milk Products Co. Ltd.,as a visiting assistant professor (1992 to 1995) and visiting associate professor (1 995at Obihiro University ofto 19 96)Agriculture and Veterinary Medicine, and asan assistant professor (1996 to 1999) andassociate professor (1999 to 2008) in theFaculty of Textile Science and Technology atShinshu University. He is engaged inphysiology,researchcryobiology,developmentalengineering in small experimental rodents,large domestic animals, and humans.ProfessorHORIE TomoakiProfessor Horie took his currentposition in 2010 after working as aresearcher at the University ofCalifornia, San Diego, and as anassistant professor on specialcontract with the Institute of PlantatScienceOkayama University, and then asan associate professor at ShinshuUniversity. His research centers onthe basic molecular physiology ofplants and plant genetic andcellular engineering based on it.Micromanipulation enablesusto handle not onlyoocytes with diameters of100 to 120 μm, but alsomuch smaller sperm cells.Intracytoplasmic injection of fishhook-shaped rat spermheads was difficult to achieve, but several clues helpedus expand its application to freeze-dried spermatozoa.My lab focuses on rice and plants in the genius Arabidopsis for molecularphysiological studies using wild-type and Na+ transporter-mutant plants.The picture shows an example of a hydroponic culture of rice plants.We analyze the target Na+ transporter that is essential for plant salttolerance in the oocyte expression system of Xenopus laevis. We areattempting to produce mutagenized Na+ transporters, which we expectto increase the salt tolerance of the host plants.asAdvancedintracytoplasmic sperm injection and somatic cellnuclear transplantation made it possible to bring themammoth back to life. Furthermore, we haverecently established pluripotent stem celllines inlaboratory rats. If these ES or iPS cells could bedifferentiated in to functional gametes, the processwould be the ultimate treatment for infertility.In my lab we study physiological aspects of early events duringfertilization in mammals as well as technical innovations involvingthe preservation and utilization of genetic resources. My studentsand I have published more than 100 research papers so fardescribing our research into haploid gametes (spermatozoa andoocytes) and diploid embryos from various mammalian species,including mice, rats, rabbits, cats, horses, buffalo, cattle, whales,and humans.I investigate the molecular mechanisms by means of whichplants protectthemselves from salinity stress (high-saltenvironments) using the experimental methods of molecularbiology, molecular genetics, and plant physiology. I hope todevelop innovative technology to generate salt-resistant cropsin order to increase agricultural yields in salt-affected areas.reproductiveonandResourcesandA microtubule network canbefromassembledcentrosome of spermatozoonimmediatelyafterfertilizationsuchtechnologiesclinicsascompaniesUsing micromanipulation to study the mechanism of fertilization and preserve, regenerate, and utilize genetic resourcesCreating new plants based on knowledge of basic plant science in order to contribute to the development of a sustainable society
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