1CRISPR/Cas9genome editing factorsDelivery system●No need of exogenous Cas9●Highly efficient genome editing●Low incidence of off-target and mosaic●High delivery of genome-edited newborns●Multiple genome editing at one time●Genome editinginadult miceCell, Organ, Individual levelFrom analysis of one gene to multiple genesMolecular PathologyCardiovascular researchProf. Takayuki Shindothe biological In our laboratory, we are always actively discussing the relationship between glycans and gastric cancer. Human gastric cancer cell line.Normal mouse (left) and obesity model mouse (ob/ob mouse) (right)LiverLiver and kidney of normal mouse and endothelial cell-specific RAMP2 knockout mouseGenome editing using maternal Cas9 and platform of next-generation model of human diseases * Functional analysis of glycans using genetically engineered mice. * Role of gastric gland mucin-specific O-glycans in preventing gastric cancer.* Investigation of various Helicobacter species in gastric diseases.It may be useful for novel molecular therapies and diagnoses of cancer andinflammation through elucidation offunctions of glycansexpressed on cell surface or secreted from cells under pathological conditions.In addition, anti-microbial drug design exhibiting an anti-microbial effect on H.pylori can be provided. H. pylori resistant to antibiotics become the problem oferadication currently.To be glycobiological specialists such as researchers in universities or publicinstitutes, private laboratories, or hospitals.・Clarification of the mechanism of disease and development of novel therapies using disease model mice and cultured cells・Roles of vasoactive peptides in vascular development, angiogenesis and cancer metastasis・Generation and analysis of gene-engineered mice using CRISPR/Cas9 ・Regenerative medical research of heart, vasculature and liverEven by the modern medicine, there still do exist incurable diseases. Mission ofmedical researchers are to elucidate their causes and develop novel therapies.Our gene-engineered mice help us to execute the mission.Our PhD students learn various medical research skills in laboratory. After thegraduation, they play leading roles as researches in universities and companies.Glycans are regarded as the third biological chains of fundamentalbiopolymers,that closelyfollowed by nucleic acids and proteins,associated with the biological phenomena such as development,immunity, infection, and cancer. We investigate the participation ofglycans in causes of various diseases using techniques such asmorphology, molecular biology, biochemistry and genetic engineering.Recently, we demonstrated that αGlcNAc protects gastric mucosaagainst Helicobacter pylori (H. pylori) infection and serves as a tumorsuppressor for differentiated-type gastric adenocarcinoma.Research subjectOutlook for researchOutlook for students after graduationIn Shinshu University, we are the only laboratory which can generate gene-engineered mice. We are developing novel technologies for genome editing(CRISPR/Cas9 method). For developing next-generation model of humandiseases, we invented novel genome editing using maternal Cas9, whichenables multiple gene modification at one time.We are studying various diseases (cancer, atherosclerosis, heart failure,liver cirrhosis and pulmonaryinfarction,diabetes,fibrosis) and have international patents for drug discoveryfailure, cerebralrenal Research subjectOutlook for researchOutlook for students after graduationNormal mouseKnockout mouseNormal mouseKnockout mouseMouse deficient in αGlcNAc Kidney Understanding mechanism of gastric cancer development through glycobiologyClarification of the mechanism of diseases and development of novel therapies~From generation to analysis of original gene-engineered mice~ 信州大学医学部医学科発行年月：２０２４年７月 発行：信州大学医学部
元のページ ../index.html#6