7ACDevelopment of microbial pesticide Bacillus thuringiensisElucidation of the mechanism of fruit body formation of basidiomycetous mushroomsBiotin producerWild typeA photomicrograph of Bacillus thuringiensisproducing spores and insecticidal crystalsFruit bodies of basidiomycetous mushrooms, Schizophyllum commune (left) and Coprinopsis cinerea (right), on agar mediaFatty acid producerFermentationtechnologyfortheproductionofusefulsubstancesisoneofthefieldsinwhichJapanhasledtheworld.WearedevelopingtheindustrialmicroorganismCorynebacteriumglutamicumasaproductionplatformnotonlyforwater-solublemetabolites,suchasaminoacidsandnucleicacids,butalsoforwater-insolublelipids.Ourcurrentinterestsinvolvemicrobialproductionofusefulfattyacidsandvitaminsderivedtherefrom(e.g.,biotin).Biotinavailableintheworldisexclusivelymanufacturedfrompetroleum,anditsmicrobialproductionhasnotbeenestablished.Weareworkingondevelopingworld’sfirstbiotechnologywithemphasizingoriginalityanduniqueidea.Keywords: Applied microbiology, Metabolic engineeringCorynebacterium glutamicum,Fatty acids and their derivatives, VitaminsWe are aiming to create an affluent and sustainable society by utilizing useful microbial resources. As the research subject, both of prokaryotic and eukaryotic microorganisms are studied by methods of molecular biology and genetic engineering.Development of innovativestrain engineering methodology with originality and practicalityDevelopment ofenvironment-friendly process independent of fossil fuelDevelopment of production base of fatty acids and sulfur-containing fatty acid derivativesCorynebacteriumglutamicumisamicroorganismthatnaturallyrequiresbiotin,animportantvitaminforourhealth.Byusinggenesfromotherbacterialspecies,wedevelopedtheworld’sfirstbiotin-nonrequiringstrainofC.glutamicum,whichproducesbiotinintheculturemedium(leftmostphoto).Wealsofoundthatonlyonemutationenablesthewild-typestraintoproducefattyacids(rightmost).InourLab,thelatesttechnologiesandBigDataoncells(genomeinformation,in-silicometabolicmaps,etc.)areusedtocreatesuperiorproductionstrains.CrystalSporeMasato IKEDAProfessor, Ph.D. KikuoSENProfessor,Ph.D.Microbial pesticides,Mushrooms,Production of useful substances,Signal transduction,Genetic engineeringFood Science and Biotechnology DivisionFood Science and Biotechnology DivisionLaboratory of Food Microbial EngineeringLaboratory ofMolecular Biology
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