Genetically modified lactic acid bacteria (gmLAB) Laboratory of Molecular BiotechnologyTakeshi SHIMOSATOProfessor, Ph.D.•Functional foods/feeds•Food immunology•Lactic acid bacteria•Mucosal vaccine•AdjuvantResearchintotheactionofprobioticsagainstinfectiousandinflammatorydiseasesandallergieshasrecentlyattractedconsiderableattentioninboththemedicalandfoodsciences.Ourcurrentresearchonthebioregulatoryfunctionoffoodproducts,particularlyinmaintainingbiologicalhomeostasisviaintestinalimmunity,hasinvestigatedtheimmunoregulatoryeffectsofprobioticlacticacidbacteriaandledtotheidentificationofactivationsequencesincludingspecificDNAmotifsinthegenomicDNA.Exploitingthispropertymayalsoproveusefulinthedesignandproductionofnewfunctionalfoods/feeds.Wearelookingforahighlymotivated,interactiveindividualtojoinateamofbasicscientistswhoseaimistodeveloppotentnewfunctionalfoods/feedsapproaches.Immunostimulatory oligodeoxynucleotides (ODNS) from lactic acid bacteria and bifidobacterium.Our group has identified immunostimulatory sequences of DNA (ISS-DNA) from immunobiotics as well as their ODN sequences. Utilization of this from the perspective of food immunology will undoubtedly lead to the invention of novel immunobiotic foods/feeds that will contribute to the prevention and amelioration of infectious, allergic, inflammatory, and autoimmune diseases.ABABCIntegrated Bioscience Division Development of mucosal vaccine using genetically modified lactic acid bacteria (gmLAB)Mucosal delivery of therapeutic proteins using gmLAB is being investigated as a new therapeutic strategy. gmLAB is a potent anti-inflammatory modulator and therefore may be effective as a mucosal vaccine.100nmODN nanocapsule Inhibitory ODN“iSG3”1% DNA50~200 nm99% CapsuleODNSupplements!?Design of super LABgmLABEvaluation of gmLAB as vaccinesMouse model of human diseaseOral routeNanoparticles for oral delivery of ODNsOur research group has identified immunomodulatory DNA sequences from immunobiotics and confirmed that several synthetic ODNs derived from these sequences maintain the immunomodulatory properties. Our approach may lead to the development of novel immunobiotic foods/feeds that will contribute to the prevention or suppression of many types of human/animal diseases.Lacticacid bacteria (LAB) are gram-positive and non-pathogenic microorganisms thathave been used as delivery vehicles for therapeutic proteins and are currentlybeing developed as mucosalvaccines through genetic engineering approaches.Wespeculated that the mucosal delivery of therapeutic proteins using gmLAB may be apromising strategy for human/animal therapy.Laboratory of Animal ReproductionYujiTAKAGIAssociateProfessor,Ph.D.•Spermatogonialstemcell•Invitrospermatogenesis•Sperm-cryopreservation•Oocyte-vitrification•AnimalbiotechnologyOurmajorinterestsaretoelucidatethephysiologicalmechanismscontrollingreproductionanddevelopmentinmammalsandtodeveloptheuseofbiotechnologyindomesticandlaboratoryanimalreproduction.Wemainlyfocusonthefollowingsubjects:(1)molecularmechanismsunderlyingmammalianspermatogonialstemcells;(2)productionofusefulanimalsbycryopreservationofsperm;and(3)vitrificationandunfrozen-preservationofmammalianoocytesandembryos.Generation of porcine spermatogonia specific (PSS-1) monoclonal antibody and efficient purification of porcine spermatogonial stem cells (SSCs) and neonatal gonocytes by using Fluorescence-Activated Cell Sorting (FACS) and Magnetic-Activated Cell Sorting (MACS). In vitro culture of porcine SSCs and prolonged growth of SSCs. Organ culture of seminiferous tubules and in vitro sperm formation. ABCDABCPSS-1 expression in neonatal porcine seminiferous tubule. Neonatal gonocytes are specifically stained by PSS-1.Ultrahigh-speed image of boar spermatozoon. We developed the observation system of sperm flagellum by using the super luminosity LED and ultrahigh-speed camera to analyze the sperm viability.Integrated Bioscience DivisionCryopreservation of boar sperm with antioxidant materials. Development of novel cryopreservation methods with a focus on injection of cryoprotective substances.Vitrification and preservation of mammalian oocytes and embryos.Purification of porcine SSCs. Cell surface of SSCs is stained by PSS-1. Live testis cell suspensions are analysis by FACS. Purified SSCs are obtained by MACS.3
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