Laboratory of Molecular cell microbiologyAkiraHOSOMIAssistant Professor, Ph.D.Keywords•Proteintransport•Proteindegradation•Yeast•Biochemistry•MolecularbiologyEukaryoticcellscontaincomplexmembranestructures.Therefore,thecellsuseasophisticatedproteintransportsystem.Recently,manypapersreported“non-classicalsecretionpathway”ineukaryote.WefoundN-glycosylationonseveralsignalpeptidedeletedproteinsinyeast.Ourmainprojectisaclarificationofnon-classicalsecretorypathwayinyeast,Saccharomycescerevisiae.Studies on intracellular protein transport and secretion in yeast.(Non-classical secretory pathway)CDYeast growth assay.Gene deletion mutants shows sensitivity.Imaging data (immunofluorescent staining)Integrated Bioscience DivisionStudies on intracellular protein degradation in yeast.(ER-associated degradation: ERAD)ABCDACApplied studies of protein transport (secretion) for cancer research.2Laboratory ofEvolutionary GenomicsShunsuke SUZUKIAssociate Professor, Ph.D.Keywords:Human Evolution,Non-coding RNAs,Retrotransposons,Genome Editing,Genomic ImprintingOurresearchesfocusontherolesoftransposableelements,whichwerespecificallyacquiredinthehumanlineage,intheevolutionofhumangeneregulationandgenomicdisorders.UsinggenomeeditingtechnologybyCRISPR/CASsystem,weproducegeneticallymodifiedhumancelllinestoinvestigatefunctionofhuman-specificretrotransposedsequences.Wealsoanalyzefunctionofnewlydiscoveredhuman-specificlongnon-codingRNAsbyoverexpressionandknockdownexperimentsinvitro.•Identification and functional analyses of novel RNA transcripts associated with human-specific retrotransposed gene copies.•Analyses of function and evolutionary impact of novel human-specific long non-coding RNAs.•Analyses of the potential role of human-specific retrotransposons in regulation of the human genome.•Analyses of the evolution of genomic imprinting in mammals.CDGenerating a genetically modified human cell line by gene manipulation. Each research project is performed by a small research group.Integrated Bioscience Division
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