Laboratory of Germ Cell Physiology and EngineeringKanako MOROHAKUAssistant Professor, Ph.D.Keywords: Oogenesis, Folliculogenesis,In vitro germ cell culture, Germ cell preservation,Animal reproductionTheobjectiveofourlaboratorymainlyfocusesonunderstandingoogenesisandfolliculogenesisinmammalianovarybygeneratinginvitroculturemethodfortheirliveimagingsystem.Backgrounds:Inmammalstheovarycontainsmorethan1millionoocytesatthemaximum.However,onlylessthan1%ofoocytesareovulatedduringreproductiveperiod,whereasmostofoocytesdegenerateinthecourseoffollicledevelopment.Thequestionsare;Howaretheovulatedoocytesselectedduringfollicledevelopment?Whatfactorsarerelatedinfolliclegrowth?Ourgoalsaretomakeclearthesequestionsandapplyournewfindingstolivestockreproductionandhumaninfertility.Research themes1.Toidentifythemolecularmechanismsofoocytedevelopmentandselectioninmammalianovaryusinglivecellimaging.ABCDBCHistologicalsectionsofmouseovarycollectedat10dayafterbirth.A:Eachoocyteisenclosedinsinglefollicle,andoocytesaregrowingconcomitantlywithfolliclegrowth.B:EnlargedfigureofA.Alotofprimordialfolliclesatthesmallestfolliclestageexistasaprimordialfolliclepool(dashedcircle).Biotechnology Division2.Todevelopanewculturemethodandchemicaldefinedmediumforinvitroovaryandfolliclecultures.3.Toidentifythemolecularmechanismsofembryoimplantationandplacentaformation.ABMost of oocytes degenerate in the course of development.How are the ovulated oocytes selected?Whatfactorsarerelatedinfolliclegrowth?Wearegoingtoidentifythemechanismsofoogenesisandfolliculogenesisusinginvitroovaryculture.B50µm34
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