農学部研究紹介英語版2020-2021
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Mycorrhiza Laboratory AkiyoshiYAMADAAssociateProfessor,Ph.D.ResearchKeywords;Ectomycorrhiza, Edible mycorrhizalmushrooms, Fungal ecologyEctomycorrhizalfungiconsistofoverten-thousandspeciesandsymbioticallyassociatewithrootsofdiversewoodyplantsinnature.Mostectomycorrhizalfungimakefruitingbodies,i.e.mushrooms.Themycorrhizalsymbiosishasbeenstudiedextensivelyintermsofplantgrowthpromotion,thecarboncycleintheecosystem,andplant-fungusco-evolution.Inaddition,cultivationofgourmetmushroomssuchasmatsutakeandtrufflesisalsopopular.Ourlaboratoryhasstudiedectomycorrhizalfungiandtheirsymbioseswithhostplantsmulti-dimensionally.Ecophysiology of ectomycorrhizal mushrooms: To establish cultivation systems of mycorrhizal mushrooms with host plants, we have focused on basic fungus–plant interaction mechanism of mycorrhizal symbiosis Fruiting of Tricholoma matsutake under natural forest condition in NaganoIn vitro mycorrhizal synthesis of T. matsutake with pine host Integrated Bioscience DivisionMorphological and anatomical characterization of ectomycorrhizas: To understand real nature of ectomycorrhizal symbiosis, we have described its structural characteristics in the diverse fungus–plant combinationsDetection, estimation and pure culture of symbiotic fungi from soil: To understand diversity of fungus–plant symbioses, we have tried to find new and rare fungal species and establish their cultures in vitro.6Laboratory of BiochemistryHiroaki KONISHIProfessor,Ph.D.Research keywords・Signal transduction・Protein phosphorylation・Knock out mouse・adaptor proteinWehaveidentifiedmanyfunctionalmoleculesactingdownstreamoftheEGF(epidermalgrowthfactor)receptortounderstandthecomplicatednetworkofitsintracellularsignalingbyphospho-proteomics.Wewillespeciallyfocusonthefunctionofthenewlyidentifiedproteinsthroughthisanalysis.ThecurrentdeterminednumberofunknownmoleculesintheEGFreceptor-mediatedsignalingpathwayisover15.Ourgoalinthenearfutureistoestablishthefunctionofeachmoleculeinthispathwaybybiochemical,molecularbiologicalandcellbiologicalmethods.ABiotechnologyDivisionGAREM projectTheGAREMfamilyofproteinsthatincludetheubiquitouslyexpressedGAREM1andbrain-specifictype,GAREM2,havebeenrecentlycategorizedasnoveladaptorproteins.CLPABP projectA PH domain-containing protein, CLPABP, plays a role in mRNA metabolism, such as its stability or instability, translation regulation, and transport in the cytosol.WDR54 projectWDR54 is a member of theWD40 repeat domain-containing protein family. This protein is cross-linked to tightly form both dimers and trimers, and that it has a regulatory effect on Erk.ABA:SchematicrepresentationoftheprimarystructureofCLPABP.Thenumberofaminoacidresiduesontheboundariesisindicated.B:CLPABPKOmicewerenormalatbirthandgrewnormallywhenyoung.However,oldmalemicepresentedafattyphenotype,similartothatseeninmetabolicsyndrome,Putative model of how cross-linked WDR54 inhibits the internalization of the activated EGF receptor. TG2 (transglutaminase 2), Ub (ubiquitin), pY (tyrosine phosphorylation)

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