Laboratory of Evolutionary Genomics Shunsuke SUZUKI Assistant Professor, Ph.D. Keywords: Human Evolution, Non-coding RNAs, Retrotransposons, Genome Editing, Genomic Imprinting Our researches focus on the roles of transposable elements, which were specifically acquired in the human lineage, in the evolution of human gene regulation and genomic disorders. Using genome editing technology by CRISPR/CAS system, we produce genetically modified human cell lines to investigate function of human-specific retrotransposed sequences. We also analyze function of newly discovered human-specific long non-coding RNAs by overexpression and knockdown experiments in vitro. •Identification and functional analyses of novel RNA transcripts associated with human-specific retrotransposed gene copies. •Analyses of function and evolutionary impact of novel human-specific long non-coding RNAs. •Analyses of the potential role of human-specific retrotransposons in regulation of the human genome. •Analyses of the evolution of genomic imprinting in mammals. C D Generating a genetically modified human cell line by gene manipulation. Each research project is performed by a small research group. Integrated Bioscience Division Laboratory of Molecular cell microbiology Akira HOSOMI Assistant Professor, Ph.D. Keywords •Protein transport •Protein degradation •Yeast •Biochemistry •Molecular biology Eukaryotic cells contains complex membrane structure. Therefore, the cells use a sophisticated protein transport system. Recently, many papers reported “non-classical secretion pathway” in eukaryote. We found N-glycosylation on several signal peptide deleted proteins in yeast. Our main project is a clarification of non-classical secretory pathway in yeast, Saccharomyces cerevisiae. Studies on intracellular protein transport and secretion in yeast. (Non-classical secretory pathway) C D Yeast growth assay. Gene deletion mutants shows sensitivity. Imaging data (immunofluorescent staining) Integrated Bioscience Division Studies on intracellular protein degradation in yeast. (ER-associated degradation: ERAD) A B C D A C Applied studies of protein transport (secretion) for cancer research. 2
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