農学部研究紹介英語版2018-2019

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Genetically modified lactic acid bacteria (gmLAB) Laboratory of Molecular Biotechnology Takeshi SHIMOSATO Associate Professor ○Functional foods/feeds ○Food immunology ○Lactic acid bacteria ○Mucosal vaccine ○Adjuvant Research into the action of probiotics against infectious and inflammatory diseases and allergies has recently attracted considerable attention in both the medical and food sciences. Our current research on the bioregulatory function of food products, particularly in maintaining biological homeostasis via intestinal immunity, has investigated the immunoregulatory effects of probiotic lactic acid bacteria and led to the identification of activation sequences including specific DNA motifs in the genomic DNA. Exploiting this property may also prove useful in the design and production of new functional foods/feeds. We are looking for a highly motivated, interactive individual to join a team of basic scientists whose aim is to develop potent new functional foods/feeds approaches. Immunostimulatory oligodeoxynucleotides (ODNS) from lactic acid bacteria and bifidobacterium. Our group has identified immunostimulatory sequences of DNA (ISS-DNA) from immunobiotics as well as their ODN sequences. Utilization of this from the perspective of food immunology will undoubtedly lead to the invention of novel immunobiotic foods/feeds that will contribute to the prevention and amelioration of infectious, allergic, inflammatory, and autoimmune diseases. A B A B C Integrated Bioscience Division Development of mucosal vaccine using genetically modified lactic acid bacteria (gmLAB) Mucosal delivery of therapeutic proteins using gmLAB is being investigated as a new therapeutic strategy. gmLAB is a potent anti-inflammatory modulator and therefore may be effective as a mucosal vaccine. 100nm ODN nanocapsule Inhibitory ODN “iSG3” 1% DNA 50~200 nm 99% Capsule ODN Supplements!? Design of super LAB gmLAB Evaluation of gmLAB as vaccines Mouse model of human disease Oral route Nanoparticles for oral delivery of ODNs Our research group has identified immunomodulatory DNA sequences from immunobiotics and confirmed that several synthetic ODNs derived from these sequences maintain the immunomodulatory properties. Our approach may lead to the development of novel immunobiotic foods/feeds that will contribute to the prevention or suppression of many types of human/animal diseases. Lactic acid bacteria (LAB) are gram-positive and non-pathogenic microorganisms that have been used as delivery vehicles for therapeutic proteins and are currently being developed as mucosal vaccines through genetic engineering approaches. we speculated that the mucosal delivery of therapeutic proteins using gmLAB may be a promising strategy for human/animal therapy. Laboratory of Animal Reproduction Yuji TAKAGI Associate Professor Spermatogonial stem cell In vitro spermatogenesis Sperm-cryopreservation Oocyte-vitrification Animal biotechnology Our major interests are to elucidate the physiological mechanisms controlling reproduction and development in mammals and to develop the use of biotechnology in domestic and laboratory animal reproduction. We mainly focus on the following subjects: (1) molecular mechanisms underlying mammalian spermatogonial stem cells; (2) production of useful animals by cryopreservation of sperm; and (3) vitrification and unfrozen-preservation of mammalian oocytes and embryos. Generation of porcine spermatogonia specific (PSS-1) monoclonal antibody and efficient purification of porcine spermatogonial stem cells (SSCs) and neonatal gonocytes by using Fluorescence-Activated Cell Sorting (FACS) and Magnetic-Activated Cell Sorting (MACS). In vitro culture of porcine SSCs and prolonged growth of SSCs. Organ culture of seminiferous tubules and in vitro sperm formation. A B C D A B C PSS-1 expression in neonatal porcine seminiferous tubule. Neonatal gonocytes are specifically stained by PSS-1. Ultrahigh-speed image of boar spermatozoon. We developed the observation system of sperm flagellum by using the super luminosity LED and ultrahigh-speed camera to analyze the sperm viability. Integrated Bioscience Division Cryopreservation of boar sperm with antioxidant materials. Development of novel cryopreservation methods with a focus on injection of cryoprotective substances. Vitrification and preservation of mammalian oocytes and embryos. Purification of porcine SSCs. Cell surface of SSCs is stained by PSS-1. Live testis cell suspensions are analysis by FACS. Purified SSCs are obtained by MACS. 3