農学部研究紹介2017-2018_英語版

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Laboratory of Cell Technology【Keywords 】(1) S. meliloti, alfalfa, symbiosis, mcpSsignal receptor, (2) transgenic plant,Menthaarvensis, Menthaaquatica,metallothionein, phytoremediationLiving organisms receive various signals from the environment and respond with signals to establish mutual relationships. The Laboratory of Cell Technology is conducting research in the following areas.(1)Characterization of the signal receptor of Sinorhizobiummelilotifor symbiosis with MedicagosativaRecently, we found that the mcpS, a signal receptor of S. meliloti, has an important role for symbiosis. One of our aims in this study is to characterize the functions of mcpSgene and of its gene product for symbiosis with alfalfa .Transgenic M. arvensiscontaining metallothioneingeneDivision of Bioscience and Biotechnology(2)Construction of transgenic mint plants by introducing metallothioneingene and their characterization.We are trying to construct a transgenic mint plants, Menthaaquaticaand Menthaarvensis, containing metallothioneingene which are expected to over-produce metallothioneine. Our goal of this study is to construct transgenic mint plants useful for phytoremediation. Red nodules formed by S. melilotion alfalfa rootAkira TABUCHIAssociate professor4Laboratory of Applied and Molecular MicrobiologyTakeshiHOSAKAAssociateProfessorActinomycetes, Antibiotic, Secondary metabolism, Ribosome engineering, Antibiotic hormesisActinomycetesproduceabundantamountsofnumerousbioactivemetabolites,includingantitumoragents,immunosuppressantsandantibioticsinparticular.Whole-genomesequencingstudiesofactinomycetestrainshaveshownthateachspeciescouldproducemanymoresecondarymetabolitesthanwereexpected.Thisindicatesthatthevastmajorityofsecondarymetabolitesremainunexpressedorbarelyexpressedunderstandardlaboratoryconditions.Thus,thereisconsiderableinterestinexploringpracticalmeanstoinducethisgeneticpotentialinactinomycetes,whichcouldresultinisolationofnovelbioactivesecondarymetabolites.Ourlaboratoryaimstounderstandthemolecularmechanismsactivatingsecondarymetabolismandtoestablisheffectivemethodsfordevelopingcrypticgeneclustersforsecondarymetabolicpathwaysinmicroorganisms.Molecular analysis of the activation mechanism for secondary metabolism of microorganisms and its application to the development of new strategies for exploring usefulbiologically active compounds.Production of useful biologically active compounds by actinomycete strains.Actinomycetesproduceabundantamountsofnumerousbioactivesecondarymetabolites.Division of Bioscience and BiotechnologyAnalysis of the molecular mechanisms of antibiotic hormesis, toward understanding the truth about antibiotics, and its applicationsfor antibiotic discovery. ActinomycetesWild-type strainMutant strain