農学部研究紹介2017-2018_英語版

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Laboratory for Regulation of PhotobiologicalProcessesMasanobu KOJIMA Professor・photobiochemistry・photoresponse・metabolomics・gene expression・protein expressionSeriouschangesinnaturalenvironmentalconditionsareworriedduetothedestructionoftheO3layerandglobalwarmingcausedbyincreasedconcentrationsofCO2intheatmosphere.Inaddition,farmersneedtoheedthedemandsofconsumersforsafefoodscultivatedusingasfewagrochemicalsandfertilizersaspossible.Undertheseconditions,“PlantFactories”,systemsforusingtechnologytogrowplantsunderhighlyregulatedconditions,arecurrentlyattractingconsiderableinterestamongpeopleworkingtodeterminethefuturedirectionoffarminginJapan.Inourlaboratory,weareusinglightemittingdiodes(LEDs),new,highlyefficientartificiallightsthatcouldbeusedforsuchplantfactories,andstudyingindetailthephotoresponsemechanismofplantsandfungigrownundertheselightconditions.1. Elucidation of PhotoresponseMechanisms of Plants and Fungi Dependent on Light Wavelength, Light Intensity, Light Stimulation Time, and light -dark cycleABCDABCLED lighting unit for plant and fungi cultivation research: the green plant is buckwheat sprout cultivated using LEDs.Oyster mushroom mycelia cultured in the dark is stimulated by blue LEDs. After light stimulation for 12 –72 h, shikimic acid is significantly accumulated depending on the time.Division of Bioscience and Biotechnology2. Production of Useful Metabolites Using Photoregulationof Metabolic Pathways of Microorganisms and Vegetables: Accumulation of Shikimic Acid and Polyphenols2Laboratory of Molecular cell microbiologyAkiraHOSOMIAssistantProfessorKeywords•Proteintransport•Proteindegradation•Yeast•Biochemistry•MolecularbiologyEukaryoticcellscontainscomplexmembranestructure.Therefore,thecellsuseasophisticatedproteintransportsystem.Recently,manypapersreported“non-classicalsecretionpathway”ineukaryote.WefoundN-glycosylationonseveralsignalpeptidedeletedproteinsinyeast.Ourmainprojectisaclarificationofnon-classicalsecretorypathwayinyeast,Saccharomycescerevisiae.Studies on intracellular protein transport and secretion in yeast.(Non-classical secretory pathway)CDYeast growth assay.Gene deletion mutants shows sensitivity.Imaging data (immunofluorescentstaining)Division of Bioscience and BiotechnologyStudies on intracellular protein degradation in yeast.(ER-associated degradation: ERAD)ABCDACApplied studies of protein transport (secretion) for cancer research.