農学部研究紹介2017-2018_英語版

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Laboratory of Food Microbial EngineeringMasato IKEDA, Ph.D. ProfessorFermentationtechnologyfortheproductionofusefulsubstancesisoneofthefieldsinwhichJapanhasledtheworld.WearedevelopingtheindustrialmicroorganismCorynebacteriumglutamicumasaproductionplatformnotonlyforwater-solublemetabolites,suchasaminoacidsandnucleicacids,butalsoforwater-insolublelipids.Ourcurrentinterestsinvolvemicrobialproductionofusefulfattyacidsandvitaminsderivedtherefrom(e.g.,biotin).Biotinavailableintheworldisexclusivelymanufacturedfrompetroleum,anditsmicrobialproductionhasnotbeenestablished.Weareworkingondevelopingworld’sfirstbiotechnologywithemphasizingoriginalityanduniqueidea.Division of Bioscience and BiotechnologyDevelopment of production base of fatty acids and sulfur-containing fatty acid derivativesDevelopment ofenvironment-friendly process independent of fossil fuelDevelopment of innovativestrain engineering methodology with originality and practicalityInourLab,thelatesttechnologiesandBigDataoncells(genomeinformation,in-silicometabolicmaps,etc.)areusedtocreatesuperiorproductionstrains.Corynebacteriumglutamicumisamicroorganismthatnaturallyrequiresbiotin,animportantvitaminforourhealth.Byusinggenesfromotherbacterialspecies,wedevelopedtheworld’sfirstbiotin-nonrequiringstrainofC.glutamicum,whichproducesbiotinintheculturemedium(leftmostphoto).Wealsofoundthatonlyonemutationenablesthewild-typestraintoproducefattyacids(rightmost).Wild typeFatty acid producerBiotin producerKeywords: Applied microbiology, Metabolic engineeringCorynebacterium glutamicum,Fatty acids and their derivatives, VitaminsLaboratory of Food Microbial EngineeringSeiki TAKENO, Ph.D. Associate ProfessorFermentationtechnologyfortheproductionofusefulsubstancesisoneofthefieldsinwhichJapanhasledtheworld.WearedevelopingtheindustrialmicroorganismCorynebacteriumglutamicumasaproductionplatformnotonlyforwater-solublemetabolites,suchasaminoacidsandnucleicacids,butalsoforwater-insolublelipids.Ourcurrentinterestsinvolvemicrobialproductionofusefulfattyacidsandvitaminsderivedtherefrom(e.g.,biotin).Biotinavailableintheworldisexclusivelymanufacturedfrompetroleum,anditsmicrobialproductionhasnotbeenestablished.Weareworkingondevelopingworld’sfirstbiotechnologywithemphasizingoriginalityanduniqueidea.Division of Bioscience and BiotechnologyDevelopmentof production base of fatty acids and sulfur-containing fatty acid derivativesDevelopment ofenvironment-friendly process independent of fossil fuelDevelopment of innovativestrain engineering methodology with originality and practicalityFatty acidproducerBiotin producerCorynebacterium glutamicumWild type InourLab,thelatesttechnologiesandBigDataoncells(genomeinformation,in-silicometabolicmaps,etc.)areusedtocreatesuperiorproductionstrains.WedevelopedC.glutamicumstrainscapableofproducingfattyacidsandbiotinforthefirsttime.MetabolicengineeringtoimprovetheproductionefficiencyisongoinginourLab.Keywords: Applied microbiology, Metabolic engineeringCorynebacterium glutamicum,Fatty acids and their derivatives, Vitamins1