農学部研究紹介2017-2018_英語版

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Laboratory of Animal ecologyAkikoTAKIIAssistantProfessorSikadeer,Asiaticblackbear,GPStelemetry,wildlifemanagement,denningbehaviorWehavebeenstudyingtheecologyofAsiaticblackbearsandsikadeerinNaganoprefecture,centralJapan.Althoughconflictsbetweentheseanimalsandpeopleareincreasing,littleisknownabouttheirecology.Theseknowledgewillprovidebetterunderstandingofthewildanimalandessentialinformationforexecutingeffectivewildlifemanagement.Myaimistoindicatethebehavior,suchasdenningecologyandsocialinteractionsofAsiaticblackbears,andseasonalmigrationofsikadeer,inordertoreduceconflictsandtoconservetheseanimals.Denning ecology of Asiatic black bears in Nagano PrefectureABCDABCSikadeergroupfeedingattheKirigamineHighland,Nagano,inwinter.Searching for the Asiatic black bear den sites. Little is known about denning in central Japan.Division of Animal ScienceSika deer behavior in mountainous region of central JapanAsiatic black bear associations at the foothills of central Japan Alps20Laboratory of Germ Cell Physiology and EngineeringKanakoMOROHAKU, PhDAssistant ProfessorKey words: Oogenesis, Folliculogenesis,In vitro germ cell culture, Germ cell preservation,Animal reproductionTheobjectiveofourlaboratorymainlyfocusesonunderstandingoogenesisandfolliculogenesisinmammalianovarybygeneratinginvitroculturemethodfortheirliveimagingsystem.Backgrounds:Inmammalstheovarycontainsmorethan1millionoocytesatthemaximum.Howeveronlylessthan1%ofoocytesareovulatedduringreproductiveperiod,whereasmostofoocytesdegenerateinthecourseoffollicledevelopment.Thequestionsare;Howaretheovulatedoocytesselectedduringfollicledevelopment?Whatfactorsarerelatedinfolliclegrowth?Ourgoalsaretomakeclearthesequestionsandapplyournewfindingstolivestockreproductionandhumaninfertility.Research themes1.Toidentifythemolecularmechanismsofoocytedevelopmentandselectioninmammalianovaryusinglivecellimaging.ABCDBCHistologicalsectionsofmouseovarycollectedat10dayafterbirth.A:Eachoocyteisenclosedinsinglefollicle,andoocytesaregrowingconcomitantlywithfolliclegrowth.B:EnlargedfigureofA.Alotofprimordialfolliclesatthesmallestfolliclestageexistasaprimordialfolliclepool(dashedcircle).Division of Animal Science2.Todevelopanewculturemethodandchemicaldefinedmediumforinvitroovaryandfolliclecultures.3.Toidentifythemolecularmechanismsofembryoimplantationandplacentaformation.ABMost of oocytes degenerate in the course of development.How are the ovulated oocytes selectedtWhatfactorsarerelatedinfolliclegrowthtWearegoingtoidentifythemechanismsofoogenesisandfolliculogenesisusinginvitroovaryculture.B50µm