農学部研究紹介2017-2018_英語版

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Genetically modified lactic acid bacteria (gmLAB) Laboratory of Molecular BiotechnologyTakeshi SHIMOSATOAssociate Professor○Functional foods/feeds○Food immunology○Lactic acid bacteria○Mucosal vaccine○AdjuvantResearchintotheactionofprobioticsagainstinfectiousandinflammatorydiseasesandallergieshasrecentlyattractedconsiderableattentioninboththemedicalandfoodsciences.Ourcurrentresearchonthebioregulatoryfunctionoffoodproducts,particularlyinmaintainingbiologicalhomeostasisviaintestinalimmunity,hasinvestigatedtheimmunoregulatoryeffectsofprobioticlacticacidbacteriaandledtotheidentificationofactivationsequencesincludingspecificDNAmotifsinthegenomicDNA.Exploitingthispropertymayalsoproveusefulinthedesignandproductionofnewfunctionalfoods/feeds.Wearelookingforahighlymotivated,interactiveindividualtojoinateamofbasicscientistswhoseaimistodeveloppotentnewfunctionalfoods/feedsapproaches.Immunostimulatory oligodeoxynucleotides (ODNS) from lactic acid bacteria and bifidobacterium.Our group has identified immunostimulatorysequences of DNA (ISS-DNA) from immunobioticsas well as their ODN sequences. Utilization of this from the perspective of food immunology will undoubtedly lead to the invention of novel immunobioticfoods/feeds that will contribute to the prevention and amelioration of infectious, allergic, inflammatory, and autoimmune diseases.ABABCDivision of Animal ScienceDevelopment of mucosal vaccine using genetically modified lactic acid bacteria (gmLAB)Mucosal delivery of therapeutic proteins using gmLABis being investigated as a new therapeutic strategy. gmLABis a potent anti-inflammatory modulator and therefore may be effective as a mucosal vaccine.100nmODN nanocapsule Inhibitory ODN“iSG3”1% DNA50~200 nm99% CapsuleODNSupplements!?Design of super LABgmLABEvaluation of gmLABas vaccinesMouse model of human diseaseOral routeNanoparticles for oral delivery of ODNsOur research group has identified immunomodulatory DNA sequences from immunobiotics and confirmed that several synthetic ODNs derived from these sequences maintain the immunomodulatory properties. Our approach may lead to the development of novel immunobiotic foods/feeds that will contribute to the prevention or suppression of many types of human/animal diseases.Lacticacid bacteria (LAB) are gram-positive and non-pathogenic microorganisms thathave been used as delivery vehicles for therapeutic proteins and are currentlybeing developed as mucosalvaccines through genetic engineering approaches.wespeculated that the mucosal delivery of therapeutic proteins using gmLABmay be apromising strategy for human/animal therapy.18Laboratory of FoodImmunologySachiTANAKAAssistantProfessorKeywords;FoodimmunologyDendriticcellsTcellsIFN-gammaNKactivityWeareplanningtoexaminetheeffectsofenvironmentalfactorsincludingfoodintakeonimmunefunctionsandelucidatemolecularmechanismsofimmuneregulation.Moreover,weareaimingfortheinvestigationofunknownimmune-modulatingeffectsofcompoundsderivedfromfoods,andidentificationoffunctionalcomponentstopreventimmunerelateddiseasessuchasinfectiousdisease,cancer,andallergy.Theseresearcheswillleadtogeneratetheapplicationofsciencetoindustryinhealthandlonglife.Identification of functional compounds and its immune-modulating activities from vegetablesInordertomaintaintheappropriateimmunefunction,itisconvenientandeffectivetointakeimmune-modulatingfoodsindailylife.WeareplanningtoidentifythefunctionalcompoundsfromvegetablesinShinshu,evaluatetheirbiologicalactivities,andelucidatethemechanismofimmuneregulation.ABCDABCDivision of Animal ScienceTheeffectofdietaryrestrictiononimmunefunctionDietaryrestrictionhasbeenshowntodelayimmunesenescence.However,theregulationofthegeneexpressionhasnotbeenfullyidentifiedafterdietaryrestriction.Wefocusonthealterationofgeneexpressionfollowingdietaryrestriction,andinvestigatehowthegenesregulateimmunefunction.