農学部研究紹介2017-2018_英語版

19/48

Laboratory of Reproductive Function in MammalsKoh-ichiHAMANOProfessorSpermmotilityThermotaxisChemotaxisHyperactivationCapacitationTheLaboratoryofReproductiveFunctionofMammalsinvestigatesthereproductivefunctioninmaleanimalstopropagatefarmanimalsandmammalsefficiently.Westudythemalegermcellsofbovines,goats,andmicetoelucidatefertilizationmechanisms.Spermmigratewiththermotaxisandchemotaxis.Sperminducehyperactivationandacrosomereactionaftercapacitation.Weanalysethermotaxis,chemotaxisandhyperactivationofmammals.Physiological mechanisms of mammalian sperm motility and capacitationABCDABCAnalysis of bull sperm motility with head motion using CASA .Computer Assisted Sperm Analysis (CASA) system.Division of Animal ScienceSignal transduction mechanisms of sperm thermotaxis and chemotaxis.Hyperactivation and acrosome reaction of mammalian sperm 14Laboratory of Animal ReproductionYujiTAKAGIAssociateProfessorSpermatogonialstemcellInvitrospermatogenesisSperm-cryopreservationOocyte-vitrificationAnimalbiotechnologyOurmajorinterestsaretoelucidatethephysiologicalmechanismscontrollingreproductionanddevelopmentinmammalsandtodeveloptheuseofbiotechnologyindomesticandlaboratoryanimalreproduction.Wemainlyfocusonthefollowingsubjects:(1)molecularmechanismsunderlyingmammalianspermatogonialstemcells;(2)productionofusefulanimalsbycryopreservationofsperm;and(3)vitrificationandunfrozen-preservationofmammalianoocytesandembryos.Generation of porcine spermatogonia specific (PSS-1) monoclonal antibody and efficient purification of porcine spermatogonial stem cells (SSCs) and neonatal gonocytes by using Fluorescence-Activated Cell Sorting (FACS) and Magnetic-Activated Cell Sorting (MACS). In vitro culture of porcine SSCs and prolonged growth of SSCs. Organ culture of seminiferous tubules and in vitro sperm formation. ABCDABCPSS-1 expression in neonatal porcine seminiferous tubule. Neonatal gonocytes are specifically stained by PSS-1.Ultrahigh-speed image of boar spermatozoon. We developed the observation system of sperm flagellum by using the super luminosity LED and ultrahigh-speed camera to analyze the sperm viability.Division of Animal ScienceCryopreservation of boar sperm with antioxidant materials. Development of novel cryopreservation methods with a focus on injection of cryoprotectivesubstances.Vitrificationand preservation of mammalian oocytes and embryos.Purification of porcine SSCs. Cell surface of SSCs is stained by PSS-1. Live testis cell suspensions are analysis by FACS. Purified SSCs are obtained by MACS.