農学部研究紹介2017-2018_英語版

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12Laboratory of Animal Functional Anatomy (LAFA)KohzyHIRAMATSUProfessorMorphologyHistochemistryGEPsystemEndocrinologyAutonomicnervoussystemLaboratoryofAnimalFunctionalAnatomy(LAFA)investigatesthemechanismsofregulatingfoodintake,thecontrolofintestinalfunctions,andtherolesofthenervoussysteminendocrineregulationbyusingimmunohistochemistryatthelightandelectronmicroscopicallevels,morphometricalanalysis,biochemicalassaysinvitroandsoon.ThekeywordofLAFAis‘EAT’.The effects of nutrients, especially protein and amino acids, on the secretion of glucagon-like peptides (GLP-1 and -2).ABCDABCUltrastructure of endocrine cells in the chicken small intestine. Arrows indicate secretory granules. N: nucleusB islet in the chicken pancreas. B islet consists of many B cells (green) and some D cells (red).Division of Animal ScienceThe neural networks regulating the food intake in the chicken hypothalamus.The innervation of chicken and ruminant pancreataby the autonomic nervous system.Laboratory of Animal Developmental GeneticsTamaoONOProfessorTeaching at Shinshu Univ. since1985.Key words: quail; chicken; development; breeding; genetics; avian biotechnology◎Utilization of avian germ cells◎Creation of new laboratory animal models for birdsABCDABC①Taking blood including primordial germ cells (future sperm or ova) from the early quail embryo②Transfusing the blood to another embryo cultured in a surrogate hen’s eggshell③Growing in the surrogate hen’s eggshell④Hatching out of the normal looking quail but may contain transfused donor-derived germ cells ⑤Donor-germ cell derived black hatchlings (right group)Division of Animal ScienceSelected publications:Mizushima, S. et al. (2014) The birth of quail chicks after intracytoplasmicsperm injection, Development, 141: 3799-3806.Kato, A. et al. (2013) Culture system for bobwhite quail embryos from the blastoderm stage to hatching, Journal of Poultry Science, 50: 155-158.Ono, T. (2000) Ex ovoculture of avian embryos. In: Methods in Molecular Biology, (eds. Tuan, R. S. and Lo, C. W.) Humana Press Inc. pp. 39-46.More info: and < http://soar-rd.shinshu-u.ac.jp/profile/en.WFfCPpkh.html>①②③④⑤Avianprimordialgermcells(PGCs)displayauniquemigrationpathwaytowardtheirtargetgonadsuchasovaryortestis,duringearlydevelopment.Assoonasthebloodvesselsformatearlystage,PGCsstarttocirculatetemporarilythroughthebloodstream,andthenthesePGCshavemigratedintothegonadsuchasovaryortestis.Thisisthepoint!Wecanobtaindonor-derivedoffspringifwetransfusethePGC-richbloodtoanotherbirdssuchaschickenandquail.Itisusefultechnologytosaveendangeredandimportantbreedofbird.Thisisachallengingresearchshouldbedonebyanimalbiotechnologyfield.