ABSTRACT

　Animal studies have shown that regular exercise prevents high-fat diet-induced obese. Autophagy has been functionally linked to adipogenesis, obesity and type II diabetes. Here, we determined if regular exercise-induced autophagy activation is sufficient to mitigate high-fat diet-induced obese. Male C57BL/6J mice were randomly assigned into 4 groups: Sedentary normal chow (Sed-NC), Sedentary high-fat diet (Sed-HF), Exercise normal chow (Ex-NC) and Exercise high-fat diet (Ex-HF). Mice in exercise group were performed voluntary wheel-running exercise for 8 weeks. Mice in high-fat diet group were fed 45% high-fat diet for 8 weeks. After exercise training periods, epididymal white adipose tissue was harvested and analyzed autophagy proteins by western blot. We showed that 8 weeks regular exercise prevented high-fat diet-induced obese, inhibited adipocyte hypertrophy and improved glucose tolerance. Autophagy flux (i.e., LC3-II protein) in Sed-HF mice was greater than Ex-NC and Ex-HF mice. These results suggest that exercise training may regulate adipose tissue homeostasis by autophagy.

DESCENTE SPORTS SCIENCE Vol.37/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE