ABSTRACT

　We have found that macrophage migration inhibitory factor (MIF) is secreted from C2C12 myotubes into culture media. In order to evaluate roles of MIF on glucose metabolism in skeletal muscle, extensor digitorum longus and soleus muscles were isolated from mice and treated with recombinant MIF in in vitro muscle incubation system. MIF itself did not affect to glucose transport in both type of muscles. However, glucose transport induced by half-max dose of insulin was diminished by co-existence of MIF in the buffer of soleus muscle incubation. These results suggest that MIF is a negative regulator of insulin-induced glucose transport in skeletal muscle. These results show that MIF is a novel myokine contributing to glucose metabolism and can be a new target molecule for prevention and treatment of diabetes.

DESCENTE SPORTS SCIENCE Vol.37/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE