Research Seeds

PDF A Basic Study on the Skeletal Muscle from the Standpoint of Sports Aptitude*Influence of Hypobaric Hypoxia and Physical Training on Fiber Composition of Skeletal Muscle in Rats

【Supercategory:7. DESCENTE SPORTS SCIENCE Subcategory:7.7 Vol.7

 Twenty four male Sprague-Dawley rats, 35 days old, were randomly assigned to one of four groups: 2 resting control groups and 2 swimming groups.
 The hypoxia control and hypoxia swimming groups were housed for one week at 550 torr, followed by 4 weeks at 460 ton.
 The sea-level control and the sea level swimming groups were housed for 5 weeks at 760 ton.
 The swimming program consisted of endurance swimming at a 30 minutes a day, 6 days a week, for 5 weeks.
 At ten weeks of age, fiber composition of their hind-limb muscles were histochemically studied. Samples of the soleus, EDL, plantaris and tibialis ant. muscles were sectioned (10μm thick) by cryostat at -20°C.
 The sections were stained with succinate dehydrogenase (SDH) and myosin adenosine triphosphatase (ATPase), and the muscle fiber were classified in to the three types: FG, FOG, SO.
 In soleus muscle, hypoxia and swimming induced significant increase in FOG fibers, and decrease in SO fibers.
 Sea-level control group had the lowest percentage in FOG fibers compared to the other groups.
 EDL and plantaris muscles had a significantly higher percentage of FOG fibers in the hypoxia and swimming groups than in the sea-level control group.
 These results obtained in the present experiments indicated that hypoxia is a triggerring factor for the conversions of muscle fiber types and the increase in oxidative capacity.

DESCENTE SPORTS SCIENCE Vol.7/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
Researcher Kazuo Itoh*1, Sadayoshi Taguchi*2
University or institution *1 Faculty of Education, Kobe University, *2 College of Liberal Arts, Kyoto University

Keywords

swimming, sea-level control, endurance swimming, hypoxia, FOG fibers