Relationship between Exercise and Health on Specific Protein Detected in Serum after Physical Exercise
【Supercategory：7. DESCENTE SPORTS SCIENCE Subcategory：7.9 Vol.9】
A specific protein which appeared in plasma after a 10 km-run was detected using two-dimensional electrophoresis, and it's properties were investigated. This specific protein could not be detected in non-exercising human plasma.
This specific protein was also detected in tri-athlon race, but it's increase did not definite.
This specific protein was detected at almost the same protein as albumin on two-dimensional gel under non-denaturing condition, but it was not adsorbed to anti-albumin-Sepharose column.
This specific protein was purified on polyacrylamide gel electrophoresis, anti-albumin affinity chromatography and isoelectrofocusing. This specific protein was detected at the position of pI 5.0, molecular weight of about 70,000 on two-dimensional polyacrylamide gel electrophoresis under non-denaturing condition, but it showed a molecular weight of 25,000 on SDS-polyacrylamide gel electrophoresis.
This protein was stained periodic acid-Shiff reagent but not with Sudan Black. This protein contained a large amount of glutamic acid (or glutamine), glycine and leucine residues, and did not contain methionine residue.
DESCENTE SPORTS SCIENCE Vol.9/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
This specific protein was also detected in tri-athlon race, but it's increase did not definite.
This specific protein was detected at almost the same protein as albumin on two-dimensional gel under non-denaturing condition, but it was not adsorbed to anti-albumin-Sepharose column.
This specific protein was purified on polyacrylamide gel electrophoresis, anti-albumin affinity chromatography and isoelectrofocusing. This specific protein was detected at the position of pI 5.0, molecular weight of about 70,000 on two-dimensional polyacrylamide gel electrophoresis under non-denaturing condition, but it showed a molecular weight of 25,000 on SDS-polyacrylamide gel electrophoresis.
This protein was stained periodic acid-Shiff reagent but not with Sudan Black. This protein contained a large amount of glutamic acid (or glutamine), glycine and leucine residues, and did not contain methionine residue.
DESCENTE SPORTS SCIENCE Vol.9/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
| Researcher | Yoshiaki Makino*1, Tsuyoki Kadofuku*1, Takumitsu Hiro*2 |
|---|---|
| University or institution | *1 Department of Biochemistry, School of Medicine, Showa University, *2 School of Dentistry Tsurumi University |
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