Individual Difference of the DHEA Concentration and its Antioxidant Activity
【Supercategory：7. DESCENTE SPORTS SCIENCE Subcategory：7.22 Vol.22】
The purpose of this study was to investigate the individual difference of the dehydroepiandrosterone (DHEA) concentration which could be associated with antioxidative capacity in young adult women.
The urinary DHEA and 11-deoxy 17-ketosteroid (deoxy 17KS) excretion of the 80 untrained women and 77 trained women were measured with gas chromatography. The DHEA and deoxy 17KS excretion of trained women were significantly lower than those of control women.
The modification of chromosomal damage in lymphocytes induced by oxidative stress were evaluated by administration of DHEA and its metabolites (DHEA sulfate, testosterone and androstenedione) by the micronucleus assay using WIL2-NS cell line.
The spontaneous and X-ray irradiated micronucleus frequencies in lymphocytes did not change by the 25μM and 2.5μM of the DHEA and DHEA sulfate.
These results suggest the possibility that in human study the serum DHEA and DHEA sulfate concentrations could be decreased by the regularly performed vigorous training, however, the antioxidative activity of DHEA could not clarified, and that in vitro study DHEA and its metabolites have no antioxidative activity.
DESCENTE SPORTS SCIENCE Vol.22/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
The urinary DHEA and 11-deoxy 17-ketosteroid (deoxy 17KS) excretion of the 80 untrained women and 77 trained women were measured with gas chromatography. The DHEA and deoxy 17KS excretion of trained women were significantly lower than those of control women.
The modification of chromosomal damage in lymphocytes induced by oxidative stress were evaluated by administration of DHEA and its metabolites (DHEA sulfate, testosterone and androstenedione) by the micronucleus assay using WIL2-NS cell line.
The spontaneous and X-ray irradiated micronucleus frequencies in lymphocytes did not change by the 25μM and 2.5μM of the DHEA and DHEA sulfate.
These results suggest the possibility that in human study the serum DHEA and DHEA sulfate concentrations could be decreased by the regularly performed vigorous training, however, the antioxidative activity of DHEA could not clarified, and that in vitro study DHEA and its metabolites have no antioxidative activity.
DESCENTE SPORTS SCIENCE Vol.22/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
| Researcher | Michiyo Kimura*1, Mitsuru Higuchi*1, Tatsuo Kato*2 |
|---|---|
| University or institution | *1 National Institute of Health and Nutrition, Division of Health Promotion, *2 National Institute of Health and Nutrition, Division of Health Promotion |
Keywords
dehydroepiandrosterone, antioxidative capacity, oxidative stress