Study of Biomarkers in Saliva as Indicators for Diagnosis of Sports Related Concussion and Return to Play
【Supercategory：7. DESCENTE SPORTS SCIENCE Subcategory：7.45 Vol.45】
ABSTRACT
Biomarkers as indicators for the diagnosis of sports related concussion have not been established. The purpose of this study was to analyze saliva samples from student American football players for microRNA and protein expression before and after concussion injury and during recovery, and to identify biomarkers in saliva as indicators of concussion diagnosis and recovery. Six samples were analyzed before concussion （Pre），nine samples duringconcussion（Post）， and three samples during concussion recovery（Rec）．MicroRNA extraction and protein mass spectrometry were performed on these samples to analyze and identify biomarkers whose expression changed at each phase. The results showed that the expression of SPRR1A （small proline-rich repeat protein1A） protein, which is considered to be related to nerve regeneration, increased significantly from Pre to Post and decreased from Post to Rec, suggesting that it may be a biomarker in saliva in sports related concussion.
DECENTE SPORTS SCIENCE Vol.45/The DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
Biomarkers as indicators for the diagnosis of sports related concussion have not been established. The purpose of this study was to analyze saliva samples from student American football players for microRNA and protein expression before and after concussion injury and during recovery, and to identify biomarkers in saliva as indicators of concussion diagnosis and recovery. Six samples were analyzed before concussion （Pre），nine samples duringconcussion（Post）， and three samples during concussion recovery（Rec）．MicroRNA extraction and protein mass spectrometry were performed on these samples to analyze and identify biomarkers whose expression changed at each phase. The results showed that the expression of SPRR1A （small proline-rich repeat protein1A） protein, which is considered to be related to nerve regeneration, increased significantly from Pre to Post and decreased from Post to Rec, suggesting that it may be a biomarker in saliva in sports related concussion.
DECENTE SPORTS SCIENCE Vol.45/The DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
| Researcher | Youhei Nakamurai*1, Hisatake Matsumoto*1, Yuki Togami*1, Jun Oda*1, Daisuke Okuzaki*2 |
|---|---|
| University or institution | *1 Department of Traumatology and Acute Critical Medicine Osaka University Graduate School of Medicine, *2 Laboratory of Human Immunology (Single Cell Genomics),WPI Immunology Frontier Research Center,Osaka University |
Keywords
Sport related concussion, Saliva, Return to play, Biomarker, Proteome analysis