ABSTRACT

　Exercise combined with food-derived factors may have significant effects on the suppression of body fat accumulation. Several trials suggested that amino acid mixtures containing alanine, arginine, and phenylalanine （AA-Mix） combined with exercise can significantly reduce abdominal fat in overweight adults and high-fat diet-induced obesity in mice. We therefore hypothesized that combining AA-Mix and exercise would significantly induce brown-like adipocyte formation, whereas either administration of AA-Mix or exercise alone would not. Administration of AA-Mix （1 g/kg） combined with exercise for 4 weeks significantly induced formation of brown-like adipocytes in inguinal white adipose tissue（ iWAT） in mice, although AA-Mix or exercise alone did not. To clarify the mechanism that exercise combined with AA-Mix induces brownlike adipocyte formation, mice were loaded a single acute exercise for 1 h after a single administration of AA-Mix, then the expression of fibroblast growth factor 21 （FGF21） and iterleukin-6 （IL-6） were examined. Plasma FGF21 concentration and the mRNA levels in the liver and iWAT did not differ between the groups after 0 and 1 h of exercise. On the contrary, plasma IL-6 concentration was significantly increased in the exercise combined with AA-Mix group compared with the exercise alone group after 1 h of exercise. These results suggest that IL-6 is involved with exercise combined with AA-Mix induced brown-like adipocyte formation. These findings demonstrate the unique effect of exercise combined with AA-Mix for inducing beige adipocyte formation.

DECENTE SPORTS SCIENCE Vol.42/The DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE