New Aspects of Oral Food Intake-Associated Hormone inIncreasing of Skeletal Muscle Mass
【Supercategory：7. DESCENTE SPORTS SCIENCE Subcategory：7.40 Vol.40】
ABSTRACT
The purpose of this study was to investigate the effects of gastric inhibitorypolypeptide （GIP）， so-called glucose-dependent insulinotropic polypeptide, andglucagon-like peptide-1 （GLP-1） receptor agonist exendin-4 （Ex4） on mousemyoblast-derived cell line C2C12. Muscle protein content in C2C12 myotubes wasincreased by 10-8 M GIP （p<0.05）．On the other hand, Ex4 （10-8 and 10-6 M） hadno impact on muscle protein content of C2C12 myotubes. GIP-associated increase ofmuscle protein content in C2C12 myotubes was observed in GIP-receptor-knockdowncells. However, GIP-associated effects on C2C12 myotubes was partially attenuated byknockdown of GIP receptor. GIP receptor knockdown itself has a stimulating effect on proliferative potential of C2C12 myoblasts. Evidences from this study strongly suggestthat digestive hormone GIP may increase or maintain skeletal muscle mass. Oral foodintake-associated digestive hormone（s）may be a potential tool for skeletal musclehypertrophy.
DECENTE SPORTS SCIENCE Vol.40/The DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
The purpose of this study was to investigate the effects of gastric inhibitorypolypeptide （GIP）， so-called glucose-dependent insulinotropic polypeptide, andglucagon-like peptide-1 （GLP-1） receptor agonist exendin-4 （Ex4） on mousemyoblast-derived cell line C2C12. Muscle protein content in C2C12 myotubes wasincreased by 10-8 M GIP （p<0.05）．On the other hand, Ex4 （10-8 and 10-6 M） hadno impact on muscle protein content of C2C12 myotubes. GIP-associated increase ofmuscle protein content in C2C12 myotubes was observed in GIP-receptor-knockdowncells. However, GIP-associated effects on C2C12 myotubes was partially attenuated byknockdown of GIP receptor. GIP receptor knockdown itself has a stimulating effect on proliferative potential of C2C12 myoblasts. Evidences from this study strongly suggestthat digestive hormone GIP may increase or maintain skeletal muscle mass. Oral foodintake-associated digestive hormone（s）may be a potential tool for skeletal musclehypertrophy.
DECENTE SPORTS SCIENCE Vol.40/The DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE
| Researcher | Katsumasa Goto, Megumi Aoshima*1, Shingo Yokoyama*2 |
|---|---|
| University or institution | *1Department of Physiology, Graduate School of Health Sciences,Toyohashi SOZO University, *2Laboratory of Physiology, School of Health Sciences,Toyohashi SOZO University |
Keywords
gastrointestinal hormone, skeletal muscle, cultured muscle cells, GIP, GLP-1