We previously reported that our novel transcutaneous CO₂ application device cause artificial Bohr effect, muscular strength augmentation, increase of mitochondria quantity, neovascularization effects. In this study, we investigated the effects of transcutaneous CO₂ application on muscle and fat volume in non-exercise condition. 25 normal adults with informed consent by written were enrolled this study. We divided the transcutaneous application for 10 min onto their right leg into five times a week and once a week, and conducted it for three months. The results showed that the quadriceps strength were significantly increased in once a week group and hamstrings strength were significantly increased in five times a week group. The muscle volume were significantly increased in both group, however, fat volume were significantly decreased slightly in once a week group. Our novel weekly transcutaneous application of CO₂ increased muscle strength and muscle volume, and decreased fat volume under nonexercise condition.

DESCENTE SPORTS SCIENCE Vol.35/THE DESCENTE AND ISHIMOTO MEMORIAL FOUNDATION FOR THE PROMOTION SPORTS SCIENCE