Lotus Base
DNA抽出
PCR増幅
DW
3 µl
2x KOD FX Neo Buffer 10
µl
2 mM dNTP 4 µl
specific primer f (10 µM)
0.6 µl
specific primer r (10 µM)
0.6 µl
lore1 5' out (10 µM) 0.6 µl
template DNA
0.8 µl
KOD FX Neo
0.4 µl
94℃ 2 min
98℃ 10 s
┐
60℃ 30 s │ 30 cycles
68℃ 1 min
┘
4℃ hold
LORE1 5' out: 5'-ACCTTGTTGCTTCAGCCATGG-3'